Polysaccharide production and degradation by bacteria in food and gastrointestinal systems

这项研究的长期目标是通过调节多糖的产生和降解来了解食物和胃肠道中微生物群落的动态。第一个主题将研究决定胞外多糖(EPS)分子质量的生物合成机制，目的是控制EPS的大小分布，以便在食品和肠道生态系统中进行测试。环境条件对基因表达的影响将使用比较转录组学来检验。这将有助于确定在食品加工过程中EPS生物合成所需的最佳条件，同时表明EPS的生产可能通过消化系统发生变化。基因编辑将有助于展示蛋白质磷酸化如何调节蛋白质功能。在第二个主题中，将调查肠道生态系统中益生菌和食源性病原体之间的相互作用。这项工作将在第三个主题中提供关于病原体排斥和肠道微生物群落稳定性的实验可验证的假说。在这个主题中，将使用人类肠道微生物生态系统模拟器来评估潜在的益生菌菌株和食品病原体在胃肠道中竞争或合作的适合性。使用离散分子质量的纤维底物进行选择性浓缩将有助于对菌株和益生菌组合的评估。此外，还将探讨益生菌和食品病原体产生的EPS在粘膜界面定植中的作用。了解益生菌和乳酸菌的基因功能和调控，对于改进酸奶和奶酪发酵剂微生物的选择，以及对食品安全和开发对人类健康有益的功能性食品至关重要。这项研究的意义在于从微生物生态学的角度了解食物结构和成分是如何影响消化的。饮食对微生物群落的组成和活性起着至关重要的作用，因此了解食品配方对生态系统的影响将有助于提高食品质量。有了这些知识，将有可能更有效地针对益生素成分，以达到对肠道微生物区系代谢活动的预期影响，并预防食源性感染。根据微生物区系组成，培养预测对特定食物的反应的专业知识，有可能开辟个性化饮食方法的前景。这项研究可以导致生产新的食品、功能食品和补充剂，以及更好地了解肠道微生物群落通过食物进行的调节。对于工业来说，研究结果可以应用于开发创新的方法，为乳清等乳制品加工副产品增加价值，这将有助于减少食物浪费。

The long-term aim of this research is to understand the dynamics of microbial communities in food and the gastrointestinal tract through the modulation of polysaccharide production and degradation. The first theme will investigate the biosynthetic mechanisms determining the molecular mass of exopolysaccharides (EPS) with the aim of manipulating EPS size distribution for testing in food and gut ecosystems. The effect of environmental conditions on gene expression will be examined using comparative transcriptomics. This will help determine the optimal conditions necessary for EPS biosynthesis in food processes, while suggesting how EPS production may change through the digestive system. Gene editing will help show how protein phosphorylation regulates protein function. In the second theme, interactions between probiotics and foodborne pathogens in the gut ecosystem will be investigated. This work will provide experimentally testable hypotheses about pathogen exclusion and gut microbiome community stability in the third theme. In this theme, the fitness of potential probiotic strains and food pathogens for competition or cooperation in the gastrointestinal tract will be assessed using the Simulator of the Human Intestinal Microbial Ecosystem. Selective enrichment using fibre substrates of discrete molecular mass will facilitate the evaluation of strains and prebiotic combinations. Furthermore, the role of EPS production by probiotics and food pathogens in colonisation of the mucosal interface will be explored. Understanding gene function and regulation in probiotic and lactic acid bacteria is essential for improving the selection of yogurt and cheese starter microorganisms, as well as for food safety and developing functional foods that have a beneficial impact on human health. The significance of this research is understanding how food structure and composition impact digestion from a microbial ecology perspective. Diet plays a crucial role in microbial community composition and activity, so comprehension of how food formulation impacts this ecosystem will help to improve food quality. With this knowledge, more effective targeting of prebiotic ingredients will be possible for attaining the desired impact on the metabolic activity of gut microbiota and preventing foodborne infections. Fostering expertise to predict responses to specific foods, depending on microbiota composition, has the potential to open up the prospect of personalised dietary approaches. This research can lead to the production of novel foods, functional foods and supplements, as well as a better understanding of gut microbe community regulation through food. For industry, the results can be applied to developing innovative ways to add value to dairy processing co-products such as whey, which will contribute to reducing food waste.