Measuring active muscle oxygenation and blood flow non-invasively during dynamic physical activity

该提案要求资金购买氧气消耗代谢率光学监测仪（MetaOx），以探索个体如何在身体活动中匹配氧气输送和氧气需求，以确定和理解个体化策略和结果如何以及为什么存在于氧气输送途径中。MetaOx是一种非侵入性监测仪，结合了活性组织氧合（频域近红外光谱）和血流（漫射相关光谱）的测量。这台监测器将是加拿大运动生理学实验室的第一台，也是加拿大国内的第二台。用途：这台监测仪将成为我研究项目的重要组成部分，并将被用于一种新颖的实验方法中，该方法侧重于研究心脏和血管如何将氧气输送与氧气需求相匹配的个体差异。当人们进行散步、跑步或骑自行车等体育活动时，氧气的输送必须与肌肉的氧气需求相匹配。心脏和血管之间的协调反应有助于促进最佳匹配，并使这些日常生活活动（ADL）得到良好的耐受。激活活动：该监视器将允许在反映ADL的动态运动（包括跑步机行走，跑步或骑自行车）期间进行活动肌肉氧合和血流量的无创测量，同时允许将实验室的知识直接转化为现实世界。目前，为了评估活跃肌肉的氧气输送，我的实验室通过动脉回声和多普勒超声无创测量肌肉血流量，而静脉血样本提供了活跃肌肉提取氧气的测量。然而，使用超声波需要关节和肢体不移动的等距收缩。这并不能反映ADL常见的动态肌肉运动的真实状态，而且还评估了“大块”肢体血流量，而不是活动肌肉。有了这个监测器，我的实验室将能够解构从口腔到肌肉的氧气输送途径，同时通过专注于独特的个体反应，实现科学方法的范式转变。影响和意义：确定个体化策略和结果在氧气输送途径中存在的方式和原因对加拿大人口来说是重要的，并且适用于整个加拿大人口，并且代表了理解血管扩张控制、个人进行体育活动的能力和对运动不耐受的脆弱性的关键缺失环节。所要求的设备的应用将创造必要的基础知识，为有针对性的、个性化的或“精确”的干预策略提供信息，类似于个性化医学的新兴领域，以改善所有加拿大人的生活，从高性能锻炼到康复设置。

This proposal requests funds to purchase an Optical Monitor of Metabolic Rate of Oxygen Consumption (MetaOx) to explore how individuals match oxygen delivery to oxygen demand during physical activity in order to identify and understand how and why individualized strategies and outcomes exist in the oxygen delivery pathway. Requested equipment: The MetaOx is a non-invasive monitor combining measures of active tissue oxygenation (frequency-domain near infrared spectroscopy) and blood flow (diffuse correlation spectroscopy). This monitor will be the first within an exercise physiology laboratory within Canada and the second within the nation. What it will be used for: This monitor will form a broad backbone of my research program and will be utilized within a novel experimental approach focusing on differences between individuals in how the heart and blood vessels match oxygen delivery to oxygen demand. When people perform physical activities like walking, running or cycling, oxygen delivery must be matched to the muscle's oxygen demand. Coordinated responses between the heart and blood vessels help facilitate an optimal matching and allow these activities of daily living (ADL) to be well-tolerated. Enabled activities: This monitor will allow for non-invasive measurement of active muscle oxygenation and blood flow during dynamic movements reflective of ADL including treadmill walking, running, or cycling while allowing direct knowledge translation from the laboratory to the real-world. Presently, to assess active muscle oxygen delivery, my laboratory measures muscle blood flow non-invasively by arterial echo and Doppler ultrasound while venous blood samples provide a measure of oxygen extraction by the active muscle. However, the use of ultrasound requires isometric contraction in which the joint and limb do not move. This does not reflect the true state of dynamic muscular movements common to ADL and also assesses `bulk' limb blood flow as opposed to active muscle specifically. With this monitor, my laboratory will be able to deconstruct the oxygen delivery pathway from the mouth to the muscle, while enabling a paradigm shift in the scientific approach by focusing on unique individual responses. Impact and significance: Identifying how and why individualized strategies and outcomes exist in the oxygen delivery pathway is important for, and applicable to, the Canadian population at large and represents a critical missing link in understanding the control of blood vessel dilation, the individual capacity to perform physical activity, and the vulnerability to exertional intolerance. Application of the requested equipment will create the foundational knowledge required to inform strategies of targeted, personalized or `precision' interventions, similar to the emerging field of personalized medicine, to improve the lives of all Canadians ranging from high performance exercise through to rehabilitation settings.