Molecular control of social memory in mice

小鼠社交记忆的分子控制

• 批准号: RGPIN-2022-04928
• 负责人: AguilarValles, Argel
• 金额: $ 2.26万
• 依托单位: Carleton University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=761435
• 关键词: Molecular; control; social; memory; mice

In mice, like most mammals, social behaviours increase survival and reproductive fitness. Social memory is a core element of social interaction, allowing recognition of stranger and familiar individuals. The CA2 subregion of the hippocampus is a crucial hub for encoding social memory and the control of social aggression. The role of CA2 in social memory has only been investigated in male mice; thus, it is unclear whether sex differences exist. Other forms of hippocampal-mediated memory (i.e., spatial memory) depend on the induction of gene transcription and mRNA translation of proteins that are just beginning to be identified. However, the molecular underpinnings of CA2-mediated social memory encoding remain poorly understood. Several genes that encode regulators of mRNA translation, such as Tsc1 (tuberous sclerosis complex 1) and Cnot3 (CCR4-NOT transcription complex subunit 3), affect the development and synaptic physiology of the hippocampus, as well as several aspects of social interaction. Despite acting on different targets, TSC1 and CNOT3 inhibit the molecular complex responsible for the initiation of mRNA translation. However, it is unclear whether they regulate social memory encoding. The long-term objective of this research program is to understand the molecular basis of social behaviours and the involvement of mRNA translation regulators in social memory encoding. During the first 5 years of this program, our short-term objectives will be to determine the transcriptomic and translatomic landscapes in CA2 excitatory neurons involved in social memory formation. Then, we will determine how TSC1 and CNOT3 are involved in regulating CA2 memory-related genes and synaptic physiology, social memory formation and social aggression. In the first part of the program, we will investigate whether the inactivation of CA excitatory neurons also affects social memory encoding in female mice. Then, we will measure transcriptomic (using single-cell RNA sequencing), and translatomic (using cell-specific ribosomal immunoprecipitation of mRNA, followed by sequencing) changes during social memory encoding in the CA2 excitatory neurons in male and female mice and how TSC1 and CNOT3 are involved in regulating translation of genes during social memory formation. Finally, we will investigate how TSC1 and CNOT3 in CA2 control social memory, aggression and CA2 synaptic responses and neuronal excitability. Overall, this program will provide an in-depth understanding of the molecular factors that control social memory formation and other forms of social behaviour controlled by the hippocampal region CA2. Furthermore, our program will open new research avenues in the molecular understanding of social behaviour.

在老鼠中，像大多数哺乳动物一样，社会行为增加了生存和生殖健康。社会记忆是社会互动的核心要素，允许识别陌生人和熟悉的人。海马体的CA 2亚区是编码社会记忆和控制社会攻击的关键枢纽。CA 2在社会记忆中的作用仅在雄性小鼠中进行了研究;因此，尚不清楚是否存在性别差异。其他形式的露营介导的记忆（即，空间记忆）依赖于基因转录的诱导和刚刚开始被识别的蛋白质的mRNA翻译。然而，CA 2介导的社会记忆编码的分子基础仍然知之甚少。 编码mRNA翻译调节因子的几个基因，如Tsc 1（结节性硬化症复合体1）和Cnot 3（CCR 4-NOT转录复合体亚基3），影响海马的发育和突触生理学，以及社会互动的几个方面。尽管作用于不同的靶点，但TSC 1和hocT 3抑制负责启动mRNA翻译的分子复合物。然而，目前还不清楚它们是否调节社会记忆编码。 这项研究计划的长期目标是了解社会行为的分子基础以及mRNA翻译调节因子参与社会记忆编码的情况。在这个项目的前5年，我们的短期目标将是确定参与社会记忆形成的CA 2兴奋性神经元的转录组和翻译组景观。然后，我们将确定TSC 1和hocT 3是如何参与调节CA 2记忆相关基因和突触生理，社会记忆形成和社会攻击。在该计划的第一部分，我们将调查CA兴奋性神经元的失活是否也影响雌性小鼠的社会记忆编码。然后，我们将测量转录组学（使用单细胞RNA测序）和translatomic（使用细胞特异性核糖体免疫沉淀的mRNA，然后测序）的变化在社会记忆编码的CA 2兴奋性神经元在雄性和雌性小鼠和TSC 1和hocT 3是如何参与调节社会记忆形成过程中的基因翻译。最后，我们将研究CA 2中的TSC 1和hocT 3如何控制社会记忆，攻击和CA 2突触反应和神经元兴奋性。 总的来说，该计划将提供控制社会记忆形成和海马区CA 2控制的其他形式的社会行为的分子因素的深入了解。此外，我们的计划将在社会行为的分子理解开辟新的研究途径。