Optimization of Enzymatic Collagen Production from Atlantic Cod Waste

大西洋鳕鱼废物中酶法胶原蛋白生产的优化

• 批准号: 571125-2021
• 负责人: Sheikhzadeh, MehdiM
• 金额: $ 1.82万
• 依托单位: Lambton College of Applied Arts and Technology
• 依托单位国家: 加拿大
• 项目类别: Applied Research and Development Grants - Level 2
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=761521
• 关键词: Optimization; Enzymatic; Collagen; Production; Atlantic

3F Waste Recovery is a Newfoundland and Labrador-based innovative biotechnology company working hand-in-hand with local fishers, farmers and foresters to extract premium quality, sustainable and natural ingredients from their by-products. Parts of locally sourced fish and shellfish that are traditionally disposed of can be processed into high-value ingredients found in everything from vitamins and supplements to cosmetics and pharmaceuticals. 3F has a licensed cleantech process for converting cod waste to highly purified collagen monomers, a full-scale functional prototype mechanical fish composter, and a fish head skinning machine that allow 3F to focus on products manufactured from the extraction of marine collagen and unique proteins from Atlantic Cod. The main objective of this project is to optimize the enzyme extraction specific to the hard water conditions in Newfoundland. First, the research team will identify the collagen yield difference when the water hardness is changed. Then the enzyme extraction would be optimized by adjusting and measuring different factors, using Design of Experiments software to help manage the optimization process. After the completion of the extraction processes, the mixture will be centrifuged, filtered, and freeze dried for the analysis of the collagen content and molecular weight range. Optimization of the process will allow 3F to continue to grow as well as inform their scaled-up production of purified collagen destined for domestic and international markets. Globally, the trend towards using natural products in health and beauty applications continues to grow. Canada has abundant natural resources that can be harnessed to fulfill this demand, but the private companies working towards this goal often do not have the necessary processing and analytical equipment needed to optimize their products. This project supports Canadian made products that have a high export potential and also supports economic development in Newfoundland. In addition, results of the project will lead to successfully adding value to waste using clean technology thereby aligning with the goals of federal and provincial governments.

3F废物回收是一家总部位于纽芬兰和拉布拉多的创新型生物技术公司，与当地渔民、农民和林农携手合作，从他们的副产品中提取优质、可持续和天然的成分。传统上处理的当地鱼类和贝类的部分可以加工成高价值的成分，用于从维生素和补充剂到化妆品和药品的各种产品中。3F拥有将鳕鱼废物转化为高纯度胶原蛋白单体的许可清洁技术流程，一个全尺寸功能原型机械鱼堆肥机，以及一台鱼头剥皮机，使3F能够专注于从大西洋鳕鱼中提取海洋胶原蛋白和独特蛋白质的产品。本课题的主要目的是优化纽芬兰硬水条件下的酶提取工艺。首先，研究小组将确定水硬度变化时胶原蛋白产率的差异。然后通过调节和测量不同因素，利用实验设计软件对优化过程进行管理。提取过程完成后，将混合物离心、过滤、冷冻干燥，用于胶原蛋白含量和分子量范围的分析。该工艺的优化将使3F公司继续发展，并为其面向国内和国际市场的纯化胶原蛋白的大规模生产提供信息。在全球范围内，在保健和美容应用中使用天然产品的趋势继续增长。加拿大拥有丰富的自然资源，可以用来满足这一需求，但致力于这一目标的私营公司往往没有必要的加工和分析设备来优化其产品。该项目支持具有高出口潜力的加拿大制造产品，也支持纽芬兰的经济发展。此外，该项目的结果将导致利用清洁技术成功地为废物增加价值，从而与联邦和省政府的目标保持一致。