转录因子OsERL1在水稻叶片形态发育中的分子调控机制研究

叶片是水稻进行光合作用主要场所，是株型的重要组成部分，与产量密切相关。目前，在水稻中虽已克隆了20多个叶形发育相关基因，但相比拟南芥，研究还不够深入，尤其是转录因子对水稻叶片极性发育中的调控机制方面研究还鲜有报道。本研究在通过EMS诱变粳稻日本晴，分离获得半显性极卷叶突变体erl1基础上，利用图位克隆获得属于HD-ZIP III基因家族的半显性水稻叶片卷曲度调控转录因子OsERL1；借助遗传学、形态学和分子生物学技术，综合CHIP-seq与RNA-seq分析结果，并通过CRISPR/Cas9进行OsERL1调控靶位点和上下游相关互作基因的遗传功能验证， 解析OsERL1在水稻叶极性发育中分子机制及其调控网络；结合杂交水稻株型育种，探讨半显性叶形调控基因OsERL1育种价值，为超级稻分子设计育种奠定坚实的材料基础并提供理论指导。

As a principle organ in rice, leaf is involved in the function of photosynthesis. Leaf size is a major determinant of plant architecture and strongly affects yield performance. To date, more than 20 genes involved in leaf morphological development have been identified and characterized, but functional research on leaf development are not in-depth studied, compare with that in Arabidopsis thaliana, especially in the regulation mechanism of transcription factors on leaf polarity development. In a previous study, a semi-dominant extreme rolled leaf mutant erl1 was obtained from EMS-induced mutagenesis of japonica rice Nipponbare and identified. Map-based cloning indicated that semi-dominant leaf rolling transcription factor OsERL1 encodes a member of class III HD-ZIP gene family. Molecular mechanism and regulatory pathway on leaf polarity development were carried out by the use of technology of genetics, morphology and molecular biology, and integrated with the analysis result of CHIP-seq and RNA-seq. CRISPR/Cas9 gene knockout technology was used to verify the regulatory target sites of OsERL1 and the genetic interactions among genes involved in pathways. It is of great material foundation and theoretical guidance to further explore the regulatory network of rice leaf morphogenesis for molecular design breeding of super rice.