水稻叶片是光合作用主要场所，其可回复性失水卷曲是对高光低湿胁迫逆境的一种适应，但分子调控机制仍不清晰。本申请拟在利用水稻光敏感卷叶突变体（photosensitive leaf 1, psl1）进行图位克隆确定候选基因PSL1的基础上，完成PSL1基因功能验证；通过室内不同光、温、湿梯度试验检测psl1及其野生型叶形变化及养分吸收速率差异；同时结合RNA-seq、酶学、生化和细胞壁糖组学分析，解析PSL1调控水稻叶形可回复性卷曲适应强光低湿逆境胁迫的分子机制。通过转录组分析比较筛选与PSL1调节的相关基因并鉴定其功能，探索水稻叶形与耐强光低湿逆境的调控网络。另外，通过PSL1与抗逆基因聚合和组织特异表达，定向改良水稻叶形，增强光能利用率，开展水稻抗逆育种利用研究。本研究有利于了解水稻体内养分吸收及代谢途径，完善水稻适应逆境胁迫的分子调控网络，为超级稻抗逆育种奠定良好的理论基础和材料基础。

Rice leaf is the most important organ for photosynthesis, and it's reversible rolling upon dehydration represents an adaptative response to the adverse stresses as such as high light intensity and low humidity, but the molecular regulation mechanisms are still not fully understood. This proposal intends to investigate the function of a candidate gene, PSL1, identified by Map-based cloning on the rice photosensitive leaf mutant, psl1. The differences of the leave shape and nutrient absorption rate between psl1 and its wild-type Nipponbare will be detected with the different light intensity, temperature and relativity thumidity gradient tests in growth chamber; RNA-seq, enzymology, biochemical and cell wall glycomics analyses will be conducted to unravel molecular mechanism by which PSL1 controls the rice leave reversible rolling in response to hydration. By transcription group analysis to find the related genes to PSL1 and identifiing its function, we will explore the regulatory networks between rice leaf-shaped and the ability to resist high light intensity and low humidity adversity stresses. In addition, PSL1 gene pyramiding with other major resistance genes and tissue-specific expression will help with oriented improvement of rice shape to increase light energy utilization efficiency , and carry out the research of rice stress tolerance breeding. This research work will benefit our understanding of the nutrient absorption, transport and metabolism in rice plants, improve the regulatory networks of rice molecules adapting to environmental adversity stresses, and build up a solid theoretical foundation and material basis for super stress tolerance breeding.