收集、分离和纯化我国红曲菌菌种资源，鉴定出7个红曲菌菌种；经筛选获高产红曲色素的紫色红曲菌Mp-41专利菌株1株（色价5340.4 U/g）。以Mp-41菌株为受体通过农杆菌介导的转化获一定量的T-DNA标签库。筛选红曲色素产量、桔霉素含量相关的突变体，采用TAIL-PCR、SON-PCR等方法克隆色素、桔霉合成关键基因，测序后通过信息生物学比对分析相关真菌（构巢曲霉、烟曲霉、米曲霉等）和突变体序列的同源性，预测其保守序列和可能的功能。构建基因缺失载体和基因恢复载体，用农杆菌介导法转化Mp-41菌株，实现关键基因阻断和过表达，并验证基因的功能。本项目研究目标是克隆2～3个新的红曲色素和桔霉素合成关键基因，掌握其功能和时空表达动态，构建低桔霉素含量高产色素的红曲菌基因工程菌株2株，实现从基因水平上对红曲色素产量和桔霉素含量进行调控。

The resources of Monascus in China were researched. Seven Monascus species were identified from samples collected around the country. A strain Mp-41 with the highest yield of pigment (5340.4 U/g) was screened out from 688 Monascus strains. This strain was used as T-DNA receptor and more than 800 inserting transformants were gained by Agrobacterium tumefaciens-mediated transformation (ATMT). Then screened pigment and citrinin yield-related mutants as sources of genes, pigment and citrinin yield-related genes would be cloned by TAIL-PCR (Thermal asymmetric interlaced PCR) and SON-PCR (single oligonucleotide nested PCR) and sequenced. Similarity of cloned genes are compared with related fungi such as Aspergillus nidulans, Aspergillus fumigatus, Aspergillus oryzae, etc. The functions of cloned genes would be predicted based on the necleotide sequence with Blast tool on line supplied by NCBI. Deletion mutants and restored mutants are constructed in order to proved the functions of genes. After construction of fusion expression vectors, target genes-GFP, the temporal and spatial expressions of genes are observed and analyzed using laser scanning confocal microscope.The objects of this research are to obtain 2-3 pigment and citrinin yield-related genes, analyze their functions and temporal and spatial expressions. Two genetic engineering Monasucus strains with highter pigment lower citrinin will be constructed. The pigment and citrinin yield of Monascus could be controlled in future by manipulating genes.