收集和分离红曲霉菌种资源685株，筛选获5株高产红曲霉色素等优质菌株；以紫色红曲霉Mp-41和红色红曲霉Mr-5专利菌株为出发菌株，建立了农杆菌介导高效转化红曲霉技术体系及克隆相关基因技术平台，构建了含5000多个转化子的T-DNA转化子库。本项目将继续扩增T-DNA转化子库，筛选分生孢子形成相关的突变体，采用TAIL-PCR、反向PCR、RT-PCR、RACE等基因克隆的技术，克隆分生孢子形成相关的基因，测序后通过信息生物学比对分析近缘真菌基因序列的同源性，预测其保守序列和可能的功能。构建基因敲除载体和基因恢复载体，用农杆菌介导等方法转化原始出发菌株，获敲除突变体和遗传恢复突变体，鉴定相关基因的功能。研究分生孢子形成相关基因的时空表达动态。本项目研究目标是克隆2～3个分生孢子形成相关的基因，掌握其功能和表达调控机制，挖掘红曲霉的功能基因，促进丝状真菌生长发育的分子机理研究。

The Monascus in China was a kind of edible and medicinal microorganism resource. 685 Monascus strains were obtained from samples collected around the country. Five quality Monascus strains were screened. The patent strains Mp-41 and Mr-5 were used as T-DNA receptors and more than 5000 inserting transformants were gained by Agrobacterium tumefaciens-mediated transformation (ATMT). The transformant library will be amplified in this study further. Then screened conidiation-related mutants as sources of genes, conidiation-related genes will be cloned by TAIL-PCR (Thermal asymmetric interlaced PCR), SON-PCR (single oligonucleotide nested PCR), iPCR, RT-PCR, RACE and sequenced. Similarity of cloned genes are compared with related fungi such as Aspergillus nidulans, etc. The functions of cloned genes would be predicted based on the necleotide sequence with blast tool on line supplied by NCBI. Deletion mutants and restored mutants are constructed in order to proved the functions of genes. After construction of fusion expression vectors, target genes-GFP, the temporal and spatial expressions of genes are observed and analyzed using laser scanning confocal microscope and qRT-PCR. The objects of this research are to obtain 2～3 conidiation-related genes, analyze their functions and temporal and spatial expressions. The conidiation mechanism of Monascus could be clarified in future. Monascus gene resources could be excavated and utilized. The research about molecular mechanism of growing development in filamentous fungi could be promoted.