Cupriavidus basilensis B-8 对木质素降解机制的研究

由于全球范围内的能源短缺，木质素生物降解的研究一直备受关注。经过几十年的努力，木质素的微生物降解机制至今仍知之甚少。在前期了解Cupriavidus basilensis B-8对木质素降解的特性，部分功能基因和作用蛋白以及下游代谢途径的基础上，本项目进一步探索木质素微生物降解机制，研究内容如下：（1）通过对醌类化合物的鉴定及其连带化学反应的分析，研究Cupriavidus basilensis B-8在木质素降解前期产生小分子可溶性氧化剂攻击木质素聚合物的机制；（2）通过蛋白质组测定和分析研究木质素降解过程中蛋白种类和表达量的变化，确定木质素降解各个阶段的功能蛋白；（3）进一步完善Cupriavidus basilensis B-8基因组数据，通过基因组分析获得上述功能蛋白的基因以及发现新的木质素降解相关基因。

Due to the worldwide shortage of energy, Research in lignin biodegradation has been focused on by lots of researchers. Microbiological lignin degradation has been studied for many years, but the knowledge of the mechanism of lignin degradation is still limited. In previous work, characterization of lignin degradation by Cupriavidus basilensis B-8, related functional genes and proteins, and catabolic pathways of downstream has been well investigated. Base on these works, the mechanism of microbiological lignin degradation will be studied in this program. There are four aspects here: 1, Cupriavidus basilensis B-8 produced small molecule soluble oxidants to attack lignin at the early stage of lignin degradation. This mechanism was studied through quinine derivatives identification and related reaction analysis; 2, Protein species and the change of their expression during the process of lignin degradation were investigated through sequencing and analysis of the proteome, and then the functional proteins were determined; 3, The data of Cupriavidus basilensis B-8 genome was further improved. The genes of functional proteins mentioned above and new gene responsible for lignin degradation were expected to be obtained。