mRNA前体选择性剪接在人类基因组中广泛存在，是调控基因表达、增加蛋白质多样性的重要机制；并且已发现许多人类疾病和mRNA选择性剪接的缺陷有关。SRSF10作为一种非典型的SR蛋白，在mRNA前体选择性剪接调控中发挥重要作用。该基因的敲除失活不但影响小鼠胚胎发育，而且敲除小鼠脂肪的形成有明显的缺陷；进一步研究发现，SRSF10 敲出的MEF细胞脂肪分化能力出现了明显的下降。我们利用SRSF10敲除的MEF细胞为研究对象，结合RNA高通量测序技术，寻找SRSF10调控脂肪分化的关键基因，在剪接调控水平上阐明SRSF10调控脂肪细胞分化的机制。

Alternative splicing is a major mechanism for regulating gene expression and contributing to protein diversity. Indeed, aberrant alternative splicing is involved in a wide range of human diseases. SRSF10 is a member of SR protein family, and plays a critical role in regulation of alternaitive splicing. Deletion of SRSF10 in mice not only affected embryonic development, but also disrupted the formation of adipose tissue in the knockout mice; further studies confirmed that SRSF10-depleted MEF cells displayed significantly decreased adipogenesis. Using SRSF10 depleted MEF cells and wt cells, we will take advantage of next-generation RNA seq to identify alternativ splicing events regualted by SRSF10 and investigate their roles in adipogenesis.