骨骼肌卫星细胞是具有增殖和自我更新能力的成肌干细胞,是骨骼肌发育和修复再生过程中的核心。研究卫星细胞活化增殖的机制,对于理解卫星细胞在骨骼肌发育或再生中的生理功能具有重要的意义。我们前期研究发现，骨骼肌敲除SRSF1的小鼠表现为骨骼肌严重发育不良；基于单根肌纤维的卫星细胞体外培养实验则证明SRSF1蛋白只在活化、增殖的卫星细胞中表达，并且SRSF1的敲除导致卫星细胞体外丧失增殖能力。本研究拟通过高通量测序结合分子生物学和细胞生物学等多种试验手段，寻找和鉴定SRSF1调控的可变剪接，揭示SRSF1及其调控的可变剪接在卫星细胞增殖中的调控机制，阐明SRSF1及其调控的可变剪接在骨骼肌发育、修复和再生中的生理功能和分子机制。

Skeletal muscle satellite cells are the muscle stem cells capable of self-renewal and proliferation, which are responsible for muscle growth through development and muscle repair after injury. Understanding their functional and regulatory mechanisms will help elucidate the role of satellite cells in muscle development and regeneration. Specific-inactivation of SRSF1 in skeletal muscle resulted in severe muscular dystrophy during postnatal development. Isolated single myofibers and in vitro culture assay showed that SRSF1 was only expressed in activated satellite cells and satellite cells lacking SRSF1 cannot proliferate as do the wildtype satellites. Here we plan to combine RNA-seq analysis with multiple molecular and cellular techniques to identifiy altentive splicing events controlled by SRSF1 and to explore the molecular mechanisms by which SRSF1 regulates proliferation of satellite cells. We also attempt to explore how SRSF1 and its alternative splicing events function during skeletal muscle growth and regeneration. These studies will provide a new paradigm with respect to the mechanism that underlies satellite cells in muscle growth, repair and regeneration.