厚壳贻贝是浙江舟山海域最主要的海水养殖品种之一。近年来，厚壳贻贝细菌病害有爆发趋势，由此带来严重的经济损失。深入阐释厚壳贻贝分子免疫调控机制，并以此为基础进行抗病分子标记辅助育种对于减轻厚壳贻贝病害发生意义重大。研究证实TLR受体介导的信号转导通路在先天免疫中发挥至关重要作用，但其在厚壳贻贝这一具有重要经济价值的海洋贝类中尚未有涉及，对于其在抗菌感染中受到非编码RNA调控的分子机制更是鲜有报道。本项目以厚壳贻贝为研究对象，拟开展TLR信号通路参与抗菌感染的作用机制研究，免疫相关miRNAs对靶基因的调控机制研究以及溶藻弧菌感染下miRNAs与TLR通路的靶向调控关系研究，最终阐释厚壳贻贝miRNAs介导TLR信号通路参与抗菌作用的分子机制。本项目的开展将有助于从本质上认识海洋无脊椎动物先天免疫信号通路的调控机制，丰富其基础分子免疫学，也可以为厚壳贻贝抗病分子育种提供靶标和理论依据。

Thick shell mussel, Mytilus coruscus is one of the most important mariculture species in Zhoushan, Zhejiang Province. In recent years, the pathogenic diseases of thick shell mussel have the tendency of outbreak, resulting in serious economic losses. Deep understanding the immunomodulatory mechanism of M. coruscus against pathogen invasions, and basing on to carry out molecular marker-assisted breeding is of great significance for the reduction the occurrence of thick shell mussel diseases. It has been comprehensively confirmed that toll-like receptor (TLR) mediated signal transduction pathway plays an crucial role in innate immunity, regrettably, there is no relevant reports has been found in M. coruscus which represented one mussel species of great economic value to date. Especially the molecular mechanism of TLR pathway regulated by non-coding RNA in antimicrobial infection is rarely involved. In this project, we will choose the typical economic bivalves M. coruscus as target species, to uncover the functional role of TLR signaling pathway involved in antimicrobial infection, as well as the regulation mechanism of immune-related miRNAs on target genes. We will also analyse the targeted regulatory relationship between miRNAs and TLR pathway in M. coruscus under the infection of Vibrio alginolyticus. Finally, the molecular mechanism of miRNAs regulating TLR signal transduction pathway involved in antimicrobial activity in M. coruscus will be elucidated. This project will be helpful to understand the regulation mechanism of innate immune pathways involved in antibacterial infection in marine invertebrates and provide molecular markers for disease resistance breeding of M. coruscus. It has important theoretical value and practical significance.