花粉表面蛋白存在于花粉外壁的花粉包被中，对花粉自身发育、花粉与柱头的授粉识别起到重要的作用。花粉表面蛋白来源于绒毡层，但是绒毡层对于花粉表面蛋白的调控机制以及各花粉表面蛋白的具体生物学功能现仍不清楚。本项目将综合应用细胞生物学、生物化学和分子遗传学等手段，具体研究：1）绒毡层调控花粉表面蛋白的分子机理。2）CRISPR创制花粉表面蛋白的单突变体与多突变体，重点分析突变体花粉的授粉和水合过程。3）花粉表面蛋白基因的表达和功能分析。通过本项目的实施，进一步揭示花粉表面蛋白的积累规律，以及这些蛋白在花粉发育、花粉和柱头识别这些过程中发挥的生物学作用。

Pollen coat, rich in pollen coat proteins (PCPs), deposits into pollen wall cavities and plays critical roles in pollen development and pollen-stigma recognition. PCPs are synthesized in tapetum, however, the regulatory molecular network in tapetum for PCPs and their functions are still unknown. In this project, using the cell biology, biochemistry and molecular genetics approaches, we will: 1) reveal the regulatory mechanism for PCPs accumulation in tapetum; 2) create the single mutants and multiple mutants by CRISPR to analyze the involvement of PCPs in pollination and hydration; 3) analyze the function of PCPs genes. This project will demonstrate the genetic regulation for PCPs and understand their functions in the processes of pollen development and pollen-stigma recognition.