白细胞共同抗原CD45剪接亚型的表达与淋巴细胞功能调节以及系统性红斑狼疮等自身免疫病的发病密切相关，CD45基因的DNA甲基化状态以及转录因子的结合对其剪接亚型的表达具有重要调控作用。我们发现CD45基因的反义链上存在自然反义转录本，干扰反义RNA能导致CD45亚型表达的改变。结合课题组前期关于反义RNA在表观遗传学水平调控基因表达的研究基础，我们提出“反义非编码RNA通过DNA甲基化调节CD45剪接亚型的表达，并可能参与自身免疫病患者淋巴细胞功能状态调控”的假说。本课题以培养的淋巴细胞以及临床系统性红斑狼疮患者外周血细胞为研究对象，检测分析反义RNA与CD45亚型的表达以及系统性红斑狼疮发病之间的相关性，探讨反义RNA调控CD45选择性剪接的可能作用机制，并为临床研究系统性红斑狼疮的发病机制提供科学依据。

The expression of leukocyte common antigen CD45 splicing isoforms is closely correlated with the function of lymphocytes and pathogenesis of autoimmune diseases. The DNA methylation and binding factors played an important function in the alterative splicing of CD45 isoforms. We found that there was a natural transcript on the antisense strands of CD45, and knocking down CD45 antisense RNA resulted the changed expression of CD45 isoforms. Based on this feature and our previous research results that antisense RNA regulated the gene expression in epigenetic level, we proposed a hypothesis that CD45 antisense RNA regulated the expression of CD45 alternative splicing isoforms through DNA methylation, and that might be involved in control of lymphocytes function in clinical patients suffering from autoimmune disease. To confirm this hypothesis, the cultured lymphocytes and peripheral blood cells in patients with systemic lupus erythematosus were applied as research model to investigate the regulatory function of antisense RNA on the alternative splicing of CD45 and to explore the possible mechanism of the regulation. Same time, this study also aims to provide research basis for the clinical study of the pathogenesis of systemic lupus erythematosus.