固相基底电喷雾电离质谱的溶剂效应与基底效应的研究

电喷雾电离质谱在食品、化学、环境及生物医药等领域具有广泛的应用。常规电喷雾电离质谱采用毛细管引导和分析制备好的液体样品，不能直接分析粘稠和固态样品等复杂样品。我们在前期工作中采用非毛细管材料如木签、铝箔、多孔聚合物和薄层色谱板以及生物组织等材料发展了新型的固相基底电喷雾电离质谱。这些方法具有操作简便和分析速度快的特点，并具有新的分析特性和功能，如实现样品的快速萃取、分离和富集等各具特色的应用。本项目拟在前期工作的基础上，系统地研究基于固相基底材料电喷雾电离质谱中的溶剂和基底材料与分析物的作用机制，阐明固相基底电喷雾电离质谱分析的内在规律，发展提高固相基底电喷雾电离质谱分析效率的辅助方法，探索发展具有快速分离功能和富集功能的电喷雾电离质谱方法。本项目的开展将有助于深入地理解固相基底电喷雾电离的基本原理，为食品、药品及毒品等实际样品的分析提供新方法，并为其他新型质谱技术的发展提供新思路。

Electrospray ionization mass spectrometry (ESI-MS) has a wide range of applications in food, chemical, environmental and biological medicine, and other fields. Capillary was commonly used for introduction of prepared liquid samples, but complex samples such as viscous and solid samples are unable to be analysed in conventional ESI-MS. In our previous work, we have developed solid-substrate ESI-MS techniques based on non-capillary materials such as wood, aluminium foil, porous polymer, thin layer chromatography plate and biological tissue, these new ESI-MS methods are conveniently and rapidly used for direct sample analysis, showed the new analytical features and functions such as rapid extraction, separation, and enrichment of analytes for versatile applications. Based on our previous work, the objectives of this project are to systematically investigate the mechanism of solvent effects and substrate effects in solid-substrate ESI-MS, and to develop new methods for improving the analytical efficiency of solid-substrate ESI-MS. The outcomes of this project will facilitate for understanding of the basic principle of solid-substrate ESI-MS. The implementation of this project also will enable us to develop simple, rapid and efficient approaches for analysis of food, pharmaceuticals and drugs, and further development of similar methods.