目标：解析基于视觉的本能恐惧反应皮层下神经环路结构和功能特征以及病理情况下发生变异的细胞机制。本研究将以野生型和DISC1基因缺陷小鼠为研究对象，整合光遗传、药物遗传学、动物活体同步光刺激多通道电生理记录、活体光纤荧光检测、单细胞测序、神经环路示踪等技术研究：1）基于视觉本能恐惧条件化的神经环路和突触可塑性机制？2）DISC1基因缺陷的动物中，处理本能恐惧反应皮层下神经环路结构和功能异常的神经环路调控机理？3）上丘内部特定亚区不同细胞种类微环路结构和功能图谱特征及其在病理情况下的变异规律？本项目将有助于我们加深理解大脑处理与物种生存相关的本能恐惧情感的神经环路结构和功能特征以及本能恐惧信息处理异常调控精神疾病发生的细胞学机制；将为研究大脑“自下而上”与“自上而下”的信息相互调控的神经基础提供新的实验依据，为研究大脑工作机制和精神疾病发生机制和治疗靶点提供新线索，具有理论、临床和经济意义。

Goals: to dissect the neural circuitry of a subcortical pathway mediating innate fear response evoked by visual stimuli, by integrating optogenetics, pharmacogenetics, in vivo multi-optrodes recording in free moving animal, optometry, single cell sequencing and viral tracing of genetically defined neural circuitry in WT and DISC1 transgenic mice. We aim to answer the following questions: 1) The neural basis of the visual-based innate fear response and how the unconditioned stimulus to be paired with a conditioned stimulus? 2）Neural circuitry dysfunction of the subcortical pathway mediating innate fear response in DISC1 transgenic mice. 3）The projectome of individual subtype of neurons within superior coliculous. This project aims to provide the neural circuitry basis of the innate fear response of survival significance, the possible cellular mechanism of neuropsychological diseases with impaired visual-based fear information processing. Further more, we will provide direct evidence for the interaction between "Bottom-Up" and "Top-Down" information flow, to shed light on the neural circuit target for neuropsychological diseases treatment.