植物先天免疫系统主要包括由胞外免疫受体介导的基础抗性和由胞内NLR免疫受体引发的小种专化性抗性两个层面，同时与其它抗病组份的动态互作调控抗病转录引发及时、适当和有效的抗病反应。已报道多个植物NLR抗病免疫受体激活后在核内行使功能，协同正调或负调抗病转录因子的作用并通过转录重编程引发抗病防卫反应。但NLR与相关组份在细胞核内的动态互作和抗病转录调控机制有待进一步的研究，NLR激活抗病的下游靶标基因及其动态调控网络有待阐明。我们将利用大麦和大麦白粉病菌的互作体系作为模式，研究大麦多个MLA抗病免疫受体与蛋白酶体及19S调节颗粒互作动态调控抗病反应的机制，建立蛋白乙酰化与多聚泛素化的关联和参与MLA介导抗病的机制；研究MLA与下游转录因子动态互作、建立抗病转录调控的动态网络。主要阐明植物NLR激活后对下游组份及抗病反应的动态调控机理。

Plant innate immune system mainly comprises two layers, the cell-surface localized PAMP-recognition receptor triggered basal immunity and the intracellular NLR receptor triggered isolate-specific immunity against pathogens. A number of plant NLRs dynamically engage downstream components that act as positive or negative transcription regulators to induce massive transcriptional reprogramming for effective immunity. However, how plant NLRs interact with other immune signaling components and what are the downstream defense-related target networks are not fully understood. Barley NLR receptors MLA trigger immunity against the powdery mildew fungus and activated MLAs interact with components of the 26S proteasome through a subunit of the 19S regulatory particle. Further understanding of the dynamic interaction of activated MLA with the 19S RP and associated acetyltransferases may premise a novel mechanism of NLR receptor-mediated immunity. We will also establish the dynamic target networks downstream of the activated NLR through interactions with transcription factors and elucidate the dynamics of NLR-mediated defense responses.