均相光致电化学生物传感新方法研究及其在重大疾病生物标志物分析中的应用

本项目拟开展高灵敏均相光致电化学生物传感新方法及其在重大疾病生物标志物分析中的应用研究。利用酶辅助原位生成量子点，实现神经退行性疾病等相关酶活性及其抑制剂的便捷均相光致电化学分析检测；基于核酸适配体技术，发展信号识别与放大新策略，实现蛋白质癌症标志物高灵敏、高选择性均相光致电化学检测；发展酶辅助信号放大新方法与新策略，建立重大疾病相关生物标志物miRNA及酶活性的高灵敏均相光致电化学分析检测新方法。构建新型均相光致电化学生物传感平台，力争实现癌症、心血管疾病、神经退行性疾病等重大疾病相关生物标志物的便捷、高灵敏、高选择性光致电化学分析检测。通过探索生物标志物表达水平及活性与疾病发生、发展之间的关联，以期为某些重大疾病的早期诊断提供新方法和新技术，具有重要的科学意义和研究价值，有望获得具有一定特色的创新性研究成果。

This project is proposed to develop highly sensitive homogenous photoelectrochemical biosensing strategies and to apply them in the analysis of biomarkers for major diseases. The activity and inhibition of enzymes that are related to diseases including neurodegenerative diseases will be detected by homogeneous photoelectrochemical method based on the enzyme product-assisted in situ formation of quantum dots. Based on DNA aptasensing techniques, target protein recognition and signal amplification will be achieved, and highly sensitive and selective homogenous photoelectrochemical analysis of protein biomarkers will be realized. Enzyme-assisted signal amplification strategies will be developed to realize highly sensitive homogenous photoelectrochemical assay of disease biomarkers such as miRNA and enzymes. A novel homogeneous photoelectrochemical biosensing platform will be built in order to realize simple, fast, highly sensitive and selective detection of biomarkers for diseases such as cancers, cardiovascular diseases, neurodegenerative diseases and so on. The relationship between biomarkers and major diseases will be investigated, and new techniques and methods for early diagnosis and clinic treatment of such diseases will be developed. Thus this project is of great scientific importance, and innovative research results are expected.