基于核酸适配体的高灵敏均相电化学蛋白质传感新方法研究及其应用

本项目拟开展基于核酸适配体的高灵敏均相电化学蛋白质传感新方法及应用研究。基于核酸适配体技术，发展高选择性蛋白质识别与信号转换新策略；采用"超级滚环扩增"、等温指数扩增等新方法，实现蛋白质识别信息的均相级联扩增与信号放大；设计基于扩散效应、接近效应等均相电化学检测新策略，构建均相电化学蛋白质传感检测平台，力争实现复杂生物体系中重大疾病相关蛋白质生物标志物的快速、稳定、高通量、高灵敏、高选择性分析检测。探索不同蛋白质生物标志物及其表达水平与重大疾病发生、发展之间的关系，以期为重大疾病如癌症的早期诊断提供新方法和新技术，具有重要的科学意义，有望获得具有一定特色的创新性研究成果。

The current project is aimed at the development of new homogenous electrochemical aptamer-based protein biosensor with high sensitivity. The highly selective discrimination strategy toward protein are developed based on the rational design of nucleic acid aptamer structure. The new methods of "super rolling circle replication" and isothermal exponential amplification are used for the homogenous cascade amplification of protein signal. The homogenous electrochemical detection strategies are designed based on the differential diffusion effect and proximinity effects, and used for the construction of homogenous electrochemical protein sensing platform.Then, the rapid,stable, high throughput, high sensitive and high selective detection toward the protein biomarkers related to the serious diseases in complex biological samples is achieved. The dependence of serious diseases on the protein biomarkers and concentration is investigated in detail. This project would provide some new methods and techniques for the early diagnosis of serious diseases. Some innovative research results are also expected with the execution of current project.