肥胖症是导致雌性不孕不育的重要因素之一，而雌性肥胖症主要影响卵母细胞的数量、卵母细胞的成熟和受精能力。先前的研究证明肥胖症可能是通过对荷尔蒙含量和脂肪因子的调节来影响卵母细胞成熟，但是到目前为止肥胖症影响卵母细胞成熟和胚胎发育的分子机制了解的并不多。本项目将集中在卵子极性方面，以诱导性肥胖小鼠和突变体缺陷型肥胖症小鼠作为模型，通过显微注射带GFP荧光标签的微丝成核因子mRNA，采用活细胞工作站、免疫荧光、免疫印迹、实时定量PCR等技术深入研究肥胖症对小鼠卵母细胞极性产生和胚胎发育的影响及其分子机制，深入了解肥胖症影响卵母细胞质量的原因，为探索并筛选肥胖症人群的生殖健康治疗药物提供理论基础和支持。

Obesity is a major cause of female infertility and sterility. Maternal obesity affects ovulated oocyte number, oocyte maturity and fertilization competence. Previous work showed that obesity altered hormone functions and adipokine expression. However,little is known about the molecular mechanism of how obesity affects oocyte maturation and embryo development. The current project will investigate the influence of obesity on oocyte polarity and related molecular mechanisms as well as embryo development in the induced obesity mouse oocytes and mutant obesity mouse oocytes by microinjection of GFP-tagged mRNA of actin nucleators, time lapse microscopy, immunofluoresence, western blot and real time RT-PCR. We will for the first time report the influence of obesity on mouse oocyte polarity and embryo development, and the findings will provide the support for furthur research of obesity-related reproductive health and the drug selection of obesity therapy.