抗根肿病基因PbBa8.1的克隆及其编码蛋白介导的抗病机制初探

根肿病是由鞭毛菌亚门根肿菌属芸薹根肿菌（原生生物）侵染引起并专性危害十字花科作物的世界性土传病害，我国油菜根肿病发病面积近千万亩且发展迅速。由于生产上缺乏抗病品种，该病已成为威胁我国油菜产业安全的头号杀手。本课题组率先培育了我国首个对根肿病具免疫抗性的新品种“华双5R”，但考虑到长期种植同一抗性品种容易导致抗性丧失，从长远来看生产上需要持久抗性品种这一重大需求，本研究拟进一步通过精细定位克隆“华双5R”的抗性基因PbBa8.1，并在此基础上发掘接种与不接种条件下在抗病反应中与PbBa8.1互作的差异蛋白，鉴定出一些病原菌分泌的效应蛋白（effector），为深入解析该抗病R蛋白所介导抗病机制以及为系统理解抗性基因抗性丧失的机制提供重要支撑，同时这些结论的获得可为通过分子设计培育对根肿病具持久抗性油菜新品种提供坚实的理论依据。

Clubroot disease caused by, Plasmodiophora brassicae is the most destructive disease affecting rapeseed (Brassica napus) production in China. Since it emergence, production losses have increased by 60% and more than 8 million mu of farm land have been abandoned because of clubroot disease infestation. Due to the lack of resistant varieties, this disease has become a major threat to the safety of China's rapeseed industry. In the last 7 years, our group at HZAU, took the lead in developing the first Clubroot resistant variety "Hua Shuang 5R", however, due to complex pathogenicity of clubroot disease and long-term cultivation of the same resistant variety is likely to lead to loss of resistance; and the need for developing broad spectrum durable resistant varieties to sustain China’s rapeseed industry. This study aims to clone the "Hua Shuang 5R" resistance gene PbBa8.1 by fine mapping approach, and on that basis further explore the relationship between PbBa8.1 and clubroot disease pathogen by looking for their differential protein interactions in order to identify key effector proteins secreted by pathogens and provide important evidence to support the study of Clubroot disease resistance mechanisms mediated by the disease resistance R proteins and to systematically dissect the mechanism underlying breakdown of Clubroot resistance genes. Overall this study has the potential to provide important theoretical basis for the future engineering of new rapeseed varieties resistant to clubroot disease.