本课题组多年来一直致力于研究微流控核酸扩增、化学发光/电致化学发光微流控检测以及芯片材料与加工等技术。本项目将在课题组先行成果的基础上，采用廉价的布材料和简单易行的微加工技术加工布基微流控芯片，布基芯片与化学发光耦合以构建一种新型布基微流控化学发光基因传感器，从而对其一些基础问题展开前瞻性研究，其内容主要包括：（1）研究用于化学发光检测的，基于聚合酶链式反应、等温核酸扩增的生物素化单链DNA制备方法；（2）研究布基微流控芯片的紫外光刻、蜡网印加工方法；（3）研究特异结合靶DNA的布基微流控传感芯片制备方法；（4）布基化学发光反应池/微流体通道设计与优化；（5）以常见食品致病菌为研究对象，采用布基微流控化学发光基因传感器进行快速、灵敏、特异的DNA检测；（6）化学发光检测器优化选择。本项目最终建成微流控生物传感新方法与技术能突破性解决现有核酸扩增、化学发光检测各自所遇到的一些难题和瓶颈。

Over the years, our research group has been devoted to the study of some microfluidic technologies such as nucleic acid amplification, chemiluminescence/electrochemiluminescence detection, as well as chip materials and fabrication. This project is based on the previous basic research results, and low-cost cloth substrate materials and easy-to-operate micromachining technologies are used to fabricate cloth-based microfluidic chips that are organically coupled with chemiluminescence to prepare a novel gene sensor. In this study, some basic, prospective problems can be studied and resolved: (1) the biotinylated, single-stranded DNA target samples, which are used for chemiluminescence detection, are prepared by using different approaches (polymerase chain reaction and isothermal nucleic acid amplification); (2) the cloth-based microfluidic chips are fabricated by wax-screen printing and UV lithography technologies; (3) the cloth-based microfluidic chips that can bind with DNA targets, are prepared; (4) the structural design of the reaction chamber/fluidic channel in the cloth-based microfluidic chip can be optimized for chemiluminescence detection of DNA targets; (5) considering the food pathogenic bacteria as the object of study, the developed cloth-based microfluidic chemiluminescence gene sensor is used for rapid, sensitive and specific detection of the DNA targets; (6) different detectors used for chemiluminescence detection can be studied and evaluated. Ultimately, this project can provide the microfluidic biosensing method and technology of the frontier of innovation for a breakthrough solution to the problems and bottlenecks encountered in nucleic acid amplification and chemiluminescence detection.