基于功能化二硫化钼构筑比率型免疫传感器对赭曲霉毒素广谱性识别的研究

食品安全关乎公众身体健康和生命安全。赭曲霉毒素是一种真菌毒素，包含多种结构类似物，在被污染的谷物中普遍共同存在，且毒性相似。目前，赭曲霉毒素A的检测较多，但其他赭曲霉毒素及代谢衍生物的检测仍较少。本项目将针对赭曲霉毒素类物质，设计和合成广谱性抗原，制备和筛选广谱性抗体，采用分子对接模型建立半抗原的选择机制，采用构效关系建立抗体与小分子的识别机制。在此基础上，研制广谱性免疫传感器，以多功能二硫化钼复合材料作为免疫传感器的基底，以DNA扩增产物构筑比率型信号，结合多元线性回归法等化学计量学手段处理数据，实现多种赭曲霉毒素的快速、灵敏的分型检测。. 本项目的完成，将为谷物中赭曲霉毒素的检测提供新技术，为真菌毒素的广谱性半抗原设计及广谱性抗体识别提供新策略，为食品中多种小分子的同时检测提供新手段。同时，通过分析赭曲霉毒素在不同谷物的分布情况，为评价其对人类健康的安全性和风险性提供理论依据。

Food security is relative with the public health and life safety. As a kind of mycotoxins, ochratoxins include many compounds with analogous structure such as ochratoxin A(OTA), ochratoxin B(OTB), ochratoxin C(OTC) and so on, which are with similar toxicity but coexist in the contaminated cereals. At present, many researches are concerned with the detection of OTA, but the investigations on other ochratoxins and corresponding derivatives are still scare. In this project, the broad-specificity antigen of ochratoxins are designed and synthesized, while the broad-specificity antibody of ochratoxins are prepared and screened. During this process, the molecular docking model are used to investigate the selection mechanism of half-antigen, while the quantitative structure-activity relationship are used to explore recognition mechanism of antibody with small molecules. Then, the broad-specificity immunosensors are constructed by using multi-functional MoS2 materials with abundant edges as the base, and the ratio-dependence from the DNA amplification as the signal. By using the corresponding chemometrics methods such as multiple linear regression and principal component analysis to analyze the data, the fast, sensitive and selective detection of ochratoxins can be achieved. . The completeness of this project will provide a new detection technology for ochratoxins, a new strategy for the design of the broad-specificity half-antigen and the recognition of broad-specificity antibody of mycotoxins, and a new analytical method for the simultaneous detection of small molecules in food. In addition, by analyzing the amount of ochratoxins in different cereals, we may provide theoretical basis for the assessment of ochratoxins on the safety and risk of human health.