骨髓间充质干细胞（MSCs）是肿瘤细胞生物学治疗的理想干细胞之一，但其在肿瘤微环境中的安全性问题日渐受关注，课题组初步已证实MSCs与肺癌A549共培养可发生恶性变化，但其机制尚待探明。本项目基于炎性信号通路失衡可诱发癌变及黄芪的扶正祛邪等理论，以Transwell小室建立MSCs与不同类型肺癌细胞株共培养体系，利用生物芯片等技术筛选诱发MSCs恶变的关键性炎性因子（或组合）及其信号通路，模拟建立诱发MSCs恶变的肺癌相关炎性因子培养体系；结合蛋白芯片、激光共聚焦技术、细胞成瘤实验等分析黄芪有效部位对炎性因子诱发MSCs恶性转化的抑制作用及其对NF-κB等炎性信号通路中促/抑恶变因子表达失衡的调控作用；揭示黄芪通过扶正祛邪、调控细胞内环境阴平阳秘而抑制炎性微环境诱发MSCs恶性变化的分子机制，并为黄芪联合MSCs防治肿瘤以及以调控细胞炎性信号通路失衡为靶点防治MSCs恶变提供理论与实验依据

Bone marrow mesenchymalstem cells (MSCs) is one kind of ideal stem cells in tumor cytological therapy, safety is concerned increasingly in the tumor microenvironment. Subject group preliminarily confirmed co-culture of MSCs and lung cancer A549 cells can produce stable genetic change. Based on the theory that the inflammatory signaling pathway imbalance can induce carcinogenesis and theory that Astragalus can culture normal Qi to expel evilness, co-culture system MSCs and different lung cancer cell lines is to be established with Transwell, technology such as biochip is to be utilized to screen for key inflammatory factors that induce malignant transformation of MSCs and for inflammatory signaling pathway, and lung cancer-associated inflammatory factor culture system is to be simulated. Protective effect of effective Astragalus parts on malignant transformation of MSCs induced by inflammatory cytokines and its regulation of expression imbalance in promoting / inhibiting malignant factors in NF-κ B and STAT3 inflammatory pathway will be analyzed by means of protein chip, confocal laser cell analysis, cell-to- tumor experiments. The molecular mechanism of Astragalus in prevention and treatment of tumor inflammatory microenvironment to induce stable genetic change of MSCs through Astragalus function in culturing normal Qi to expel evilness, regulating intracellular environment to get balance of yin and yang will be revealed. The theoretic and experimental basis of combining Astragalus and MSCs in treating tumor and regulating inflammatory signaling pathway imbalance will be provided to prevent malignant change of MSCs.