寒冷是高寒地区畜禽的一种普遍应激因素，如何提高机体耐寒能力是生产者关注的热点问题。机体对寒冷的适应叫冷适应，指经过反复的冷刺激，使机体对低温产生适应性，从而促进机体耐寒性增强。本研究拟在肉鸡的生长早期给予不同的间歇式冷刺激，采用体况、生产性能、抗氧化能力、免疫力和抗应激性等综合指标作为筛选冷适应建立的参数，获得使肉鸡建立冷适应的合理方案。本研究结果在生产上为实施适宜的冷刺激训练提供科学依据。.采用现代组学技术，包括转录组学和蛋白组学技术，对冷适应组和对照组的肝脏组织和下丘脑组织进行差异基因和差异蛋白的筛选，并对其进行生物信息学分析，筛选出对建立冷适应可能有重大影响和重要调控作用的基因和蛋白质以及有较大研究价值的Pathway，并将蛋白组与转录组研究结果进行关联分析，实现mRNA和蛋白质的互补和整合，系统全面的揭示肉鸡建立冷适应的分子机制。

Cold stress is very common for livestock and poultry in the cold areas. How to enhance animal’s ability to tolerate cold temperatures is the hotspot problem in production. Cold acclimation is a process by which animal acquire a tolerance to cold after a period of exposure to low temperatures. In order to get the reasonable scheme to establish cold acclimation in broiler, we apply different intermittent cold stimulus at the early growth stage of broilers and use aggregative indicator including body condition, production performance, oxidation resistance, immunity and anti-stress capability as the parameter to evaluate the cold acclimation. Results of this study will provide a scientific basis for appropriate cold stimulation training in poultry production..We will use modern omics technologies including proteome and transcriptome to identify differentially expressed genes and proteins in liver tissue and the hypothalamus tissues of broilers from cold acclimation group and control group. The bioinformatics analysis will be carried out on differentially expressed genes and proteins. Some genes and proteins that have a significant impact and important regulatory role will be screened and the pathway that may have greater research values for brolier's cold acclimation will be found. These results will be able to reveal the mechanism underlying cold acclimation from two aspects of gene and protein. Using combined analysis on the results of transcriptome and proteome, we can obtain results that is complementary and integration of mRNA and protein. Results of this study will contribute to fully reveal the molecular mechanism of cold acclimation.