微量矿物离子在骨组织再生与修复过程中发挥无可替代的作用，但对其启动生物功能途径的作用机制尚未见文献报道。本项目拟解决的关键科学问题是微量骨矿物离子-骨性蛋白配体的形成及其在仿生液晶态环境中与骨性细胞的相互作用机制。由于微量骨矿物离子种类繁多，本项目拟以Sr(II)与钙敏感骨性蛋白的结合为核心，创新性地在分子水平上揭示Sr(II)与钙敏感骨性蛋白特异识别、结合形成配体的热力学和动力学规律；更重要的是，本项目还模仿骨性矿物离子体内生物学微环境，系统研究Sr(II)-骨性蛋白配体的生化形态在仿生微环境中影响骨性细胞应答的机制，探明Sr(II)-骨性蛋白作用配体在仿生液晶态微环境中介导骨性细胞响应和细胞内相关信号转导通路。在上述研究的基础上，建立骨矿物离子在骨组织再生或修复过程中的作用机制研究的新思路和新方法，为探讨其它骨矿物离子在骨再生或修复过程中的作用机制开辟一个新途径。

Trace mineral ions play a key role in bone regeneration and repair. However, the mechanisms involved in the mobilization of their biological functions have not been reported. The key scientific issues to be studied in this project are the mechanism of the formation of strontium-osteogenic protein ligands (SOPL) and their interactions with bone cells in biomimetic liquid crystal microenvironments. As there is a great variety of trace mineral ions in bone tissue, in this project, by taking the combination of strontium ions and calcium-sensing osteogenic proteins as the pivot, we reveal the thermodynamic and dynamic laws of the formation SOPL through specific recognitions and combinations at the molecular level. More importantly, we simulate the biological microenvironment of bone mineral ions in vivo, and systematically investigate bone cellular responses induced by SOPL in the biomimetic microenvironment. We illuminate the molecular mechanisms of cellular responses and signaling pathway mediated by SOPL in the biomimetic liquid crystalline microenvironment. On the basis of these studies, we establish a novel strategy and method for exploring the mechanisms of mineral ions in bone regeneration and repair, paving the way for understanding the roles of other functional bone mineral ions in bone regeneration and repair.