量子点的生物学效应及其机理的同步辐射研究

本课题拟选取水相合成的镉系量子点，发展和应用基于同步辐射软线的X射线显微技术，对量子点在细胞内的精确分布和细胞的形貌变化进行高分辨成像，定量地分析量子点与自噬相关蛋白和关键亚细胞器的共定位信息，并结合使用分子生物学和生物化学的手段对自噬相关的信号转导通路进行研究，揭示量子点引发细胞自噬的具体机理，从而有望进一步阐明量子点的细胞学效应，为量子点的生物学应用奠定基础。

In this project, we intend to explore the detailed subcellular distribution pattern of several kinds of cadmium-based QDs with element-sensitive, nanometer-scale resolution imaging approaches at SSRF。We will focus on the QDs-induced cellular morphological change and quantitative information of co-localization between QDs and key proteins and organelles in the process of autophagy. In combination with research using biochemical and molecular biological approaches, these study will provide new insight into the interaction between QDs and essential cellular signaling pathways like autophagy。