T-ALL的浸润转移是威胁患者生命的主要因素之一。我们前期研究发现CCR9的高表达介导了T-ALL的浸润与转移，NOTCH信号通路参与CCR9高表达的调控，然而促进CCR9高表达的调控机制尚未明了。TCONS_00024295是申请者在GEO数据库中筛选得到的一条与CCR9呈高度正相关的全新lncRNA，并在T-ALL细胞系和临床样本中验证了其相关性，目前国内外尚未见报道。为明确lncRNA TCONS_00024295调控CCR9高表达的机制，本课题拟以天然高表达CCR9的T-ALL细胞系及临床样本为研究对象，运用RNA-FISH、流式细胞术、RNA pull-down等技术，探讨TCONS_00024295通过PURB/GATA-2上调CCR9的表达，进而促进T-ALL浸润转移的调控机制，为T-ALL的防治提供一种新的思路。

Metastasis and invasion of T-ALL are the main risk factors threatening the lives of T-ALL patients. Our previous study has reported that high expression of CCR9 contributed to metastasis and invasion of T-ALL, and NOTCH signal pathway could be involved in the regulation of CCR9 expression. However, the underlying mechanism of CCR9 high expression is still unclear. Based on the bioinformatics analysis of GEO database, we have selected a new lncRNA TCONS_00024295 which has highly positive correlation with the expression level of CCR9. Consistently, we have identified that there is a positive correlation between TCONS_00024295 and CCR9 in T-ALL cell lines and clinical samples. However, it has not been reported by any research group. In order to uncover the underlying mechanism of TCONS_00024295 in regulating CCR9 high expression, we will use CCR9 naturally highly-expressed T-ALL cell lines and clinical samples, and explore the mechanism of CCR9 up-regulation via TCONS_00024295/PURB/GATA-2 complex by RNA-FISH, flow cytometry, RNA pull-down and other technologies. This study will provide a new idea for the prevention and treatment of T-ALL metastasis and invasion.