母乳内源性多肽PDC89用于肥胖防治的应用基础研究

PDC89是我们借助质谱技术发现的、功能未知的母乳内源性多肽。前期发现，PDC89具有抑制人脂肪源性间充质干细胞增殖的功能，给ob/ob小鼠饮水口服有显著的减重效果，提示PDC89具有潜在的肥胖防治作用；进一步揭示其作用与抑制JAK-STAT信号通路有关，已证实STAT3与人脂肪源性间充质干细胞增殖密切相关。研究拟评估PDC89在不同物种脂肪源性间充质干细胞、不同肥胖动物模型的功能一致性，论证其在肥胖防治中的可能性；借助JAK-STAT信号通路激动剂OSM、 Pull-down实验、Co-IP方法，系统阐明其调控JAK-STAT信号通路的分子机制。研究可望为肥胖防治提供新手段。

PDC89 is a breast milk-derived endogenous peptide with unknown functions identified by mass-spectrometric technique. We found that PDC89 inhibited the adipose-derived mesenchymal stem cells proliferation. Oral administration of PDC89 for ob/ob mouse had an obvious effect on losing weight, indicating that PDC89 may play a potential role in obesity prevention and treatment. It further revealed that its effects were associated with inhibition of JAK-STAT signal pathway. It has been reported that STAT3 is closely related to the proliferation of adipose-derived mesenchymal stem cells. This research intends to evaluate the consistency of the function of PDC89 in mesenchymal stem cell of different species and in different obese animal models and further evaluate its possibility in obesity prevention and control. This research systematically elucidates the molecular mechanism of how PDC89 regulating JAK-STAT signal pathway through JAK-STAT signal pathway activated agent OSM, the Pull-down experiment and Co-IP method. It is expected to provide new insights for obesity prevention and treatment.