硫化氢（H2S）是继NO 与CO 之后，在动植物体中被发现的第3种功能性气体信号分子。H2S在动植物体内，被证明具有“Do It All（全能）”的功能。H2S在果蝇和线虫中具有延长寿命、提高耐热性、耐旱性和生殖能力的功能。H2S调控家蚕生长发育至今尚没有报道。前期我们已经初步探明合适浓度的H2S处理可以有效延长5龄幼虫的龄期和蛹期、诱导中肠收缩压的增强、增加全茧量、茧层量和产卵量。本项目开展H2S调控家蚕的生长发育研究，确定H2S合理的作用浓度，明确H2S调控家蚕的生化和代谢过程，鉴定H2S调控家蚕发育时间的关键信号通路。项目的实施，率先开展H2S气体信号分子对家蚕生长的调控，对于丰富气体信号分子调控生命体的理论和应用具有一定的意义；对于把H2S开发为养蚕的生长调节剂提供理论基础，实现养蚕业的高产、高质和稳产具有重要的支撑作用。

Hydrogen sulfide (H2S) is the 3rd gaseous signaling molecule that was found after NO and CO.H2S displayed Multiple important functions just called ‘Do It All’. Exposure to H2S could prolong the lifespan, improve heat resistance, drought tolerance and reproductive capacity of Drosophila melanogaster and Caenorhabditis elegans. There are no reports about the regulation of H2S on the growth and development of silkworm to date. Our previous research demonstrated that the appropriate concentration of H2S treatment can effectively prolong the 5th instar larvae and pupal stage, induce the increase of the systolic pressure in the midgut, increase the amount of the whole cocoon, cocoon shell and eggs. In present study, H2S will be used to regulate the growth and development of silkworm; and then, the reasonable concentration of H2S treatment of silkworm will be determined; the corresponding biochemical, metabolic changes and the key signaling pathways of H2S regulating the development time of silkworm also will be identified. The implementation of the project will be the first time to analyze the regulations of H2S on the growth of silkworm, which is significant important to enrich the theory and application of gas signal molecules in regulating life, will provide a theoretical basis for further development H2S for sericulture growth regulator, and will play important roles to achieve high yield, high quality and stable production of sericulture.