高危型人乳头瘤病毒(HPVs)是多种恶性肿瘤的直接诱因，发展新一代抗HPV制剂迫在眉睫。HPV次要衣壳蛋白L2的抗原是由序列连续且高度保守的氨基酸组成的“线性表位”，可以直接被嫁接到其它载体上而发挥功能，是研发新一代抗HPV制剂的焦点。基于多金属氧簇(POMs)的潜在生物学应用，我们拟将它们引入到HPV衣壳蛋白L1或L2抗原表位肽(L2pep)的多重组装调控中, 拟在三个不同层次开展杂合组装调控：(1) L2pep与POMs; (2) L2pep-POMs组装体与L1五聚体; (3) 以类病毒粒子(VLPs)及其杂合组装体为构筑基元的更高级别组装。开发稳定性好、免疫性高且广谱的新型生物-无机抗病毒材料。重点研究多级组装中的主要驱动力及关键调控因素，揭示内在分子机理和机制；考察由组装带来的原有各组装基元生物学性能的协同增强性和材料新功能，为抗HPVs药物的研发提供新的理论依据和技术支持。

Infection of high risk human papillomavirus (HPVs) causes various malignant tumors, it is urgent need to develop the new generation of anti-HPV agents. The linear epitopes in the minor capsid protein L2 are the highly conserved and succession extended amino acids which can be graft on other backbone easily but keep their immunity well, and therefore, it has become a new focus to develop a broad-spectrum anti-HPV agents. The potential bio-applications of polyoxometalates (POMs) push us to introduce them to monitor the assemblies of HPV L1 protein and L2 peptides (L2pep), to create highly stable, broad-spectrum anti-HPV new materials. For that, multi-scaled assemblies will be performed between (1) L2pep and POMs to create L2pep-POMs; (2) L2pep-POMs and L1 pentamer (L1-p) to generate L2pep-POMs@VLP; (3) VLPs and/or L2pep-POMs@VLP to stabilize the final products before use. Both the driving forces and key factors to regulate the multi-scaled assemblies will be focused to reveal the intrinsic mechanism and involved kinetic at molecular level. The functions of the newly created materials, particularly the newly developed and synergistic enhancement ones from assembly will be paid special attention in performance. The present study will provide new theoretical basis and technical supports for the research and development of anti-HPV drugs in future.