重要致灾病害水稻条纹叶枯病是由灰飞虱传播水稻条纹病毒（RSV）引起的。目前，灰飞虱传毒的分子机制还不清楚。前期发现，RSV的pc2蛋白及其受体蛋白GN-Rp对RSV侵染和跨越介体中肠屏障具有重要贡献，但机制不详。本项目拟从传毒决定因子pc2入手，筛选pc2受体蛋白GN-Rp，克隆GN-Rp基因并制备抗体，多种方法验证pc2与GN-Rp之间的互作，从蛋白质水平解析GN-Rp三维结构与传毒功能之间的关系；通过RNA干扰、抗体封闭技术、饲毒传毒实验、组织细胞定位等研究，从生物学水平明确pc2及GN-Rp在灰飞虱的传毒（获毒）过程中的作用。同时将GN-Rp基因开发为鉴别灰飞虱传毒能力的生物标记，并制备灰飞虱不可获毒的水稻材料。本项目在揭示RSV跨越介体中肠屏障的分子机理的同时，为水稻条纹叶枯病的绿色可持续防控提供了技术手段。

Rice stripe virus (RSV) has been reported to cause severe rice stripe disease in rice fields in China, and RSV is transmitted by small brown planthopper (Laodelphax striatellus Fallén, SBPH). At present, the transmission mechanisms of RSV by SBPH remain unclear. Our preliminary study showed that RSV-pc2 and its receptor protein GN-Rp had important contributions to RSV infecting and escaping from midgut barrier, but the mechanism is unknown. The project was planned to take transmission determinants pc2 as the breakthrough, the pc2's receptor protein GN-Rp was screened, GN-Rp gene was cloned, and its antibody was prepared, then the interaction between two proteins was verified using various methods, analyzing the relations between the three-dimensional structure of GN-Rp and its function for RSV transmission in the protein level. Afterward, RNAi, antibodies blocking, virus-feeding experiments and tissue localization were performed to reveal the functions of pc2 and GN-Rp in RSV transmission (acquisition) by SBPH in a biological level. Meanwhile, the GN-Rp gene as bio-marker for RSV transmission in SBPH was also developed, and rice cultivar that SBPH had difficulties in acquiring RSV was prepared. At the same time revealing the molecular mechanisms that RSV escaped from midgut barrier, the project could provide techniques and means for sustainable control of rice stripe disease.