在规模化养殖过程中，仔猪很容易受到许多因素的影响，引起机体氧化应激，导致肠道屏障氧化损伤，生长性能降低。如何维持仔猪肠道健康已成为现代生猪生产的难题。本研究应用ELISA、免疫组织化学、实时荧光定量PCR、细胞流式术、激光共聚焦显微镜技术、ussing chamber和蛋白印迹等技术考察仔猪血清、肠道粘膜以及IPEC-J2氧化与抗氧化相关指标，并分析肠道和IPEC-J2的通透性和TER以及紧密连接蛋白的表达与分布，考察德氏乳杆菌缓解氧化应激介导肠道屏障功能损伤；采用Btk和Nrf-2抑制剂，以及RNA干扰技术敲除IPEC-J2中TLRs、Btk、Nrf-2的表达，考察TLR-Btk-Nrf-2信号通路在调控肠道氧化还原平衡和肠道屏障功能中的作用，揭示德氏乳杆菌缓解仔猪肠道屏障氧化损伤作用机制，为乳酸菌制剂的开发和在畜牧生产中应用提供理论依据。

Piglets are readily subjected to various factors, causing host oxidative stress, dysfunction of intestinal barrier function, and finally resultant lower growth performance in pig industry. How to maintain intestinal health is a key problem in modern pork production. This study aimed to evaluate lactobacillus delbrueckii ameliorate oxidative stress mediated intestinal barrier disorder in piglets through analyzing oxidive and anti-oxidative parameters in serum, intestinal mucosa and IPEC-J2, intestinal permeability and trans-epithelium electrical resistant (TER) and tight junction proteins expression and distribution in intestine and IPEC-J2 by using enzyme-linked immuno sorbent assay (ELISA), immunohistochemical techniques, real-time PCR, confocal fluorescent microscope, ussing chamber, and western blot. Using Btk and Nrf-2 specific inhibitors and RNA interference technique to knock-down TLRs, Btk, Nrf-2 expression investigate the role of TLR-Btk-Nrf-2 signaling pathway in modulating intestinal redox homeostasis and barrier function, unveiling the mechanism of lact delbrueckii alleviate intestinal barrier oxidative damage, which form the theoretical basis for the development of lact delbrueckii-based probiotic and its application in the livestock production.