抗病基因（R基因）在植物基因组中常以多基因聚集的方式形成复合位点，而且已知的绝大多数功能性R基因均出自复合位点。那么，功能性R基因是如何从复合位点中演化出来的呢？本项目以大豆中两个抗大豆花叶病毒复合位点（Rsv1与Rsv3）为主要研究对象，探索抗性基因的功能化机制。首先，我们拟调查Rsv位点在野生大豆种群以及多个栽培品种中的变异模式；然后，利用病原株系对所有大豆材料进行抗性功能鉴定，比较Rsv位点在抗、感群体间的演化模式差异；最后，通过杂交定位、文库构建以及高通量测序等手段，有效确定Rsv位点上的多个功能抗SMV基因，回溯它们的功能演化过程，总结其分子机制。此研究将帮助我们更好地理解复合R基因位点的自然变异模式，探索不同选择压力下复合位点功能R基因的演化机制，为合理利用乃至人工构建持久、有效的抗病基因奠定基础。

Within plant genomes, resistance genes (R genes) often cluster together and most currently-known functional R genes are derived from such complex loci. How did functional R genes evolve from complex loci then? To answer this question, in this project, we plan to investigate two soybean complex loci, Rsv1 and Rsv3, which often contain functional R genes resistant to soybean mosaic virus (SMV). First, the variation patterns of Rsv loci in multiple soybean wild populations and cultivars will be examined. Second, several SMV strains will be employed to survey the resistance phenotypes of all used soybean material. Differences in evolutionary pattern of Rsv genes will be checked between resistant group and sensitive group. Last, through mapping, library constructing and screening, and high-throughput sequencing, we try to identify some functional SMV-resistant R genes in Rsv complex loci and to backtrack their functionalization processes. This project would help us better understand the evolutionary pattern and functionalization process of R genes in complex loci, which will lay a good foundation for constructing durable R genes in the future.