调节性B细胞在棘球蚴感染中的作用及机制研究

包虫病严重影响新疆农牧民身体健康和经济发展，但对其免疫机制认识不足阻碍了包虫病疫苗的研制。最近研究发现存在一种新型调节性B细胞（B10细胞），通过产生抑制性细胞因子IL-10发挥免疫调节作用，但对其在包虫病中的作用尚未阐明。本项目根据前期实验结果，研究包虫病患者手术前后及临床转归患者B10细胞变化规律，观察棘球蚴感染小鼠B10细胞和相关细胞因子在感染不同阶段的动态变化，分析包囊周围B10细胞和IL-10表达与包囊形成及炎症反应的关系，探讨B10细胞与棘球蚴感染免疫病程的相关性；通过包虫抗原EgB、Eg95体外刺激和树突状细胞共培养及特异性抗体阻断CD40/CD40L信号通路对B细胞增殖的影响，探讨包虫不同抗原和DC诱导B10细胞的产生机制；阐明这一新型调节性B细胞在棘球蚴感染中的免疫调控及其作用机制，为提高疫苗保护性效果和以调节性B细胞为靶点免疫干预治疗包虫病开拓新的思路和途径。

Echinococcosis affects seriously farmers health and economic development in xinjiang. However, further study of echinococcosis vaccines has been hinded by poor knowledge to infection immune mechanism of Echinococcus granulosus. Recently studies show a new regulatory B cells (B10 cells) has been found, but their role in echinococcosis has not been clarified. According to the experimental results we have got, we will research the change regulation of B10 cells in Echinococcosis patients before and after surgery and clinical outcome respectively, then we will observe the dynamic change of B10 cells and related cell factors in the early, middle and late of infection of Echinococcus granulosus in mice, and detect expression of B10 cells and IL-10 around a cyst, which will be analyzed in the cyst formation and inflammatory response, probe the correlation between B10 cells and infection immune of Echinococcus granulosus. Meanwhile,through the influence on stimulation in vitro of antigen EgB, Eg95 and DC and specific blocking CD40 / CD40L signal pathway to the proliferation of B10 cells.Then we will analyse the effect on B10 cells differentiation by different antigen and DC, clarify the immune regulation function of the new B cells in infection of Echinococcus granulosus, which will develop new ideas and approaches for protective effect of vaccine of immune intervention echinococcosis as B10 cells for targets.