传统的毒理学评价方法己远不能满足对生殖毒性化学物快速筛检和机制研究的需要，随着动物实验减少、替代、优化 "3R"原则的提出，建立快速、敏感的生殖毒性替代模型已成为现代毒理学发展的时代要求。秀丽隐杆线虫因其具有遗传背景清晰、进化上高度保守、结构简单、生命周期短等特点，近年来在毒理学研究中已受到关注。本研究利用典型生殖毒物构建秀丽隐杆线虫生殖毒性模型及其评价指标体系，探讨秀丽隐杆线虫生殖毒理学替代模型用于生殖毒性化学物筛检和机制研究的可行性，通过分析比较国家当前重点推荐的高毒有机磷类替代农药毒死蜱对线虫和小鼠的生殖毒性，为其应用于生殖毒性化学物的筛检提供依据。

The long-time and widespread usage of pesticides produces the potential health hazards on human populations. However, the traditional reproductive toxicity methods don't meet the demand of screening the potential reproductive toxicant and the mechanism study.With the development of "3R" principle (Reduction, refinement and replacement), it is the demand of development of modern toxicology to establish a rapid and sensitive reproductive toxicity alternative model. The study aimed to establish the C. elegans reproductive toxicity alterative model and evaluating indicators firstly. Further, the application value of C. elegans reproductive toxicity alternative model was explored by the comparative analyses between chlorpyrifos C. elegans reproductive toxicity alternative model and chlorpyrifos rodent toxicity model. The study provides evidence to the establishment of reproductive toxicity alternative model for the screening of potential reproductive toxicants and the mechanism study of reproductive toxicants.