人类、鼠和植物等研究证实：转座子插入多态（TIP）是具有诸多优点的新型分子标记，在基因精细定位、遗传进化、品种鉴定等研究中具有很高的应用价值，但目前猪基因组TIP研发不足，一定程度上制约了分子育种进程。本研究通过构建猪混合基因组转座子多重插入富集文库，进行靶向高通量测序，获得猪基因组转座子插入位点信息后进行群体遗传学分析，建立猪基因组TIP数据库，评估TIP对猪遗传进化和品种形成的影响。然后注释TIP在基因组上的分布特征，获得与功能基因相关联的候选TIP。在此基础上，筛选一些可能影响重要经济性状的TIP，进行表型关联研究，筛选与表型关联性较强的位点，探究转座子插入对关联基因活性影响的分子机制。项目预期能够建立猪全基因组TIP数据库，获得若干有育种价值的TIP，发表论文2-4篇，申请专利1-2项。TIP研发将推动猪功能基因学研究和分子育种的进程，加深对猪起源进化和品种形成的理解和认识。

The study of human, mice and plants confirmed the transposon insertion polymorphism (TIP) are new molecular marker with many advantages, and which represents a high application value in the research fields of gene fine mapping, genetic evolution, breed identification, but the research and development of pig genome TIP was rare in the past, which, to some extent, restricted the swine molecular breeding process. In this study, the mixing porcine genomic insert multiple transposon libraries were constructed, and then the transposon insertion site sequence data were obtained by targeted high-throughput sequencing. The population genetic analysis was further conducted to setup the pig genome-wide TIP database and to assess the application value of TIP in the research of pig evolution, breed formation. Then the TIP distribution in the genome was annotated to identify the TIPs inserted in or around functional genes. On this basis, the candidate TIPs associated with genes, which may affect important economic traits, were selected for phenotype association studies, and the strong correlation TIPs with phenotype were remained for further explore the molecular mechanisms of the gene activity changes due to the transposon insertion. This project is expected to get the pig genome TIP data, obtain some valuable TIPs in pig molecular breeding, publish 2-4 papers, and apply 1-2 patents. The research and development of pig TIPs will promote the development of pig functional genomics and molecular breeding process, deepen the understanding of the origin and evolution of swine, and the breed and strain formation.