目前，对非编码RNA的生成和功能研究比较深入，但对其降解和清除的了解非常有限。我们前期研究中发现血管生成素（angiogenin，ANG），一个多功能的核糖核酸酶A超家族成员，不仅可以下调一系列miRNAs，而且以内切酶的方式降解成熟miR-141，可能是第一个被发现的参与miRNA降解的核酸内切酶。为此，本项目拟在进一步解析ANG降解成熟miR-141方式和分子机制的基础上，利用深度测序技术全面探索ANG是否参与其它非编码RNA的降解和/或加工过程，揭示其分子机制，并明确ANG降解miR-141的方式和机制是否具有普遍性。然后，选择受ANG降解的2条代表性非编码RNA，探索其本身及降解产物在ANG介导的肿瘤血管新生中的作用。通过本项目研究，不仅将明确ANG在非编码RNA加工和/或降解代谢中的作用，而且将揭示一些受其调控的非编码RNA的生物学功能，进一步加深对ANG功能及其作用机制的了解。

The biogenesis and function of noncoding RNAs have been widely studied, but their degradation and elimination are poorly understood. Our previous studies have shown that angiogenin (ANG), a member of RNase A superfamily, not only downregulates a series of miRNAs, but also degrades the mature miR-141 by its endoribonucleolytic activity. Therefore, ANG might be the first mammalian endoribonuclease which plays a key role in mature miRNA degradation. In this project, we will further investigate the digestion manner of mature miR-141 catalyzed by ANG and its underlying mechanism of action, and then examine the role of ANG in other noncoding RNA degradation and/or processing by the second generation deep sequencing technology. Hopefully, we might be able to elucidate the common mechanism of ANG in noncoding RNA degradation. Next, we will choose two representative noncoding RNAs which were directly degraded by ANG, and explore the roles of both ncRNAs and their degradation products in ANG-mediating tumor angiogenesis. We believe our project could elucidate the role of ANG in noncoding RNA degradation and/or processing, and unveil biological functions of some ANG-regulating noncoding RNAs, thus deepening our understanding on the functions and mechanisms of ANG.