舌鳞癌是口腔最常见恶性肿瘤，多数患者在确诊时已发生淋巴结转移。由于缺乏有效治疗手段，临床预后较差。近年来研究发现葡萄糖转运蛋白1（Glut-1）可增加肿瘤细胞葡萄糖代谢，加速肿瘤生长。但Glut-1与舌鳞癌侵袭和转移的相关报道甚少。我们前期研究发现：Glut-1在舌鳞癌中过度活化；Glut-1抑制剂WZB117可降低舌鳞癌细胞的增殖、迁移和侵袭能力；通过白花丹素（PLB）阻断PI3K/Akt通路可下调Glut-1表达；PLB和WZB117均可下调MMP-2表达。据此，我们推测Glut-1在舌鳞癌侵袭和转移过程中起重要作用，而PLB可能通过下调Glut-1抑制舌鳞癌侵袭和转移。本研究拟采用组织芯片、过表达及RNA干扰等技术，通过体内、体外实验明确Glut-1在舌鳞癌侵袭和转移中的作用和分子机制，阐释PLB抑制舌鳞癌侵袭和转移的分子机制，为抗癌药物研发提供新的思路。

Tongue squamous cell carcinoma (TSCC) is the most common cancer in oral cavity. Most of diagnosed TSCC cases have occurred lymph node metastasis and there is a lack of effective therapies, resulting in poor prognosis. Recently, studies found that glucose transporter 1 (Glut-1) can increase glucose metabolism in tumor cells and accelerate tumor growth, but there are rare researches on the association of Glut-1 with the invasion and metastasis of TSCC. Our previous study found that Glut-1 was overexpressed in TSCC; WZB117 (Glut-1 inhibitor) suppressed the proliferation, migration and invasion of TSCC cells; Plumbagin (PLB) down-regulated the expression of Glut-1 via inhibiting PI3K/Akt pathway; Both PLB and WZB117 all down-regulated MMP-2 expression. Accordingly, we speculate that Glut-1 plays an important role in promoting the invasion and metastasis of TSCC, and that PLB may inhibit the invasion and metastasis of TSCC via down-regulating Glut-1. This study intends, in vitro and in vivo, to clear the role and mechanism of Glut-1 in the invasion and metastasis of TSCC and to clarify the effect and molecular mechanism of PLB on inhibiting the invasion and metastasis of TSCC. It is expected to provide a new idea in research and development of anti-cancer medicine.