家蚕核型多角体病毒对家蚕细胞周期的调控机制研究

病毒和宿主相互作用的研究对病毒增殖复制机制和宿主抗性机制的解析具有重要的生物学意义。本项目以家蚕核型多角体病毒（BmNPV）调控家蚕细胞周期进程为研究对象，探究杆状病毒和宿主相互作用机制，可为病毒侵染机制解析和家蚕核型多角体病防控提供理论支撑。项目拟通过流式细胞术等方法分析BmNPV作用于家蚕细胞后细胞周期的变化特征，确定同步化细胞对病毒DNA复制的影响；进而根据BmNPV阻滞细胞周期时空特征和对应时期杆状病毒转录表达特征筛选BmNPV调控宿主细胞周期的关键病毒因子和周期相关蛋白，并分析其作用；最后，通过基因定点敲除敲入、酵母双杂交和组学等方法研究杆状病毒利用细胞周期阻滞促进自身增殖复制机理。通过本项目的研究，可为解析杆状病毒调控宿主细胞周期的机制和家蚕核型多角体病的防控提供支撑。

The study of the interaction between the virus and the host has important biological significance for the identification of the virus molecular biology and the analysis of the host resistance mechanism. In this project, Bombyx mori nucleopolyhedrovirus (BmNPV) arresting the cell cycle is used as research object to analysis how baculovirus regulates the cell cycle process of the host and the mechanism of the interaction between baculovirus and host. First of all, this project will analysis the effect of the host cell cycle on BmNPV viral production by flow cytometry and other methods and determine the impact of synchronized cells on the replication of DNA virus; then screen the key viral factors and cyclin related proteins that BmNPV regulates the host cell cycle based on the BmNPV blocking the cell cycle Spatiotemporal characteristics and baculovirus transcription expression characteristics, and analyze their roles. Finally, various methods, including gene knockout and knockin, yeast two hybrids, and omics and so on will be used to research the mechanism how baculovirus uses cell cycle arrest to promote viral production. These results can provide theoretical support for analyzing the mechanism of baculovirus regulating host cell cycle and the prevention and control of the BmNPV.