球孢白僵菌是在害虫生物防治中具有广阔应用前景的丝状真菌。本课题以该菌为材料，围绕过氧化物酶体自噬参与菌体氧化应激这一新发现，探求丝状真菌应对氧化胁迫的新机制。首先，从自噬相关基因ATG11介导过氧化物酶体自噬出发，通过蛋白互作和基因功能鉴定，逐步解析介导过氧化物酶体自噬的选择性自噬受体（SAR）及其靶蛋白，揭示决定过氧化物酶体自噬专一性和选择性的遗传基础。其次，通过分析氧化胁迫条件下的过氧化物酶体差异蛋白质组，明确氧化胁迫导致过氧化物酶体生理失衡的原因，揭示细胞器受损和引发自噬的机制。第三，揭示SAR及其靶蛋白响应氧化胁迫的转录调控机制。最后，揭示翻译后修饰机制调控SAR及其靶蛋白互作的模式。本项目将首次揭示丝状真菌的过氧化物酶体自噬途径及该途径参与氧化应激的分子机制。这不仅为进一步认识昆虫病原真菌环境适应性和致病机制提供新的理论，而且为菌株改良和应用技术进步提供新的策略。

Beauveria bassiana is an important filamentous entomopathogenic fungus, has a great potential for the biological control of insect pests. Oxidation tolerance is a determinant factor for fungal environmental adaptation and virulence when undergoing the saprophytic and parasitic growth. Our preliminary researches indicated that B. bassiana autophagy-related gene 11 (BbATG11) mediates pexophagy during fungal response to oxidative stress and contributes to fungal virulence. This study is sought to decipher the mechanisms involved in the pexophagy during fungal response to oxidative stress. First, to identify the potential selective autophagy receptor (SAR), the yeast two-hybrid system and related techniques are adopted to characterize the interacting proteins of BbATG11p. The actual SAR genes are determined by generation of gene disruption mutants. Then, on the basis of the identified SAR, its potential interaction proteins on peroxisome are identified by the above mentioned strategy. Second, the effects of oxidative stress on peroxisome are revealed by analyzing the organellar proteome after oxidative stress. Third, the mechanisms of interaction between SAR and its interaction proteins are revealed by the biochemical and genetic techniques. Additionally, their transcriptional regulators are identified via the yeast one-hybrid system. Finally, all results are used to develop a network of the BbATG11-dependent pexophagy in B. bassiana under oxidative stress. This study highlights for the first time that pexophagy contributes to oxidation tolerance of filamentous mycopathogen, and adds the new knowledge to the molecular mechanisms involved in fungal resistance to oxidative stress. Such theory provides a novel strategy to improve the environmental fitness and efficacy of entomopathogenic fungi as biocontrol agents.