纳米生物材料进入体内后将首先吸附蛋白，在其表面形成新的"纳米-生物界面"即蛋白晕，使其获得新的生物学身份。本项目旨在研究具有高度有序的三维支化结构的树枝状大分子可控修饰超顺磁性四氧化三铁纳米粒（SPION），运用蛋白质组学的方法探索带有不同表面基团（电荷）和靶向配体的SPION对在体外细胞培养基、细胞外基质和血浆中形成的蛋白晕组成、含量及蛋白构象的影响，进而研究SPION/蛋白晕纳米复合物对细胞的粘附、胞吞/胞吐途径、细胞标记效率及活体细胞正常机能的影响，探索蛋白晕在SPION胞吞过程中与细胞受体的相互作用，并进一步研究其对SPION生物分布的影响。项目的实施将为深入理解和认知纳米-生物界面（蛋白晕）及其与细胞相互作用，指导更为成熟和多元化的SPION表面修饰，使其在生理环境中更好地发挥生物功能，获得更佳的生物相容性，加快推进多种临床应用，为疾病的早期诊断、治疗以及组织修复做出贡献。

Once nano-biomaterials enter into the body, the first happened thing is the protein adsorption onto the surface to form a new " nano-bio interface", that is, "protein corona", which will endow the materials with a new biological identification in vivo. The project aims to study the superparamagnetic iron oxide nanoparticles (SPION) controllably modified with the highly ordered three-dimensional branching structure of dendrimers. Take the use of proteomics method, to explore the influence of the modified SPION with different surface groups (charge) or targeting ligands to the chemical composition, content and protein conformation etc. of protein corona, which is formed in cell culture matrix solution, extracellular matrix or plasma protein. And then to study the effect of SPION/corona compounds on the cell adhesion, endocytosis/spit-out, cell labeling efficiency, and the normal function of living cells, and the interactions with the cell receptors. Then further study the impact of SPION biological distribution in vivo. The implementation of the project will be in-depth understanding the nano-bio interface (protein corona) and its interaction with cells, which will provide more guidance for the diversified SPION surface modification, and to accelerate a variety of clinical applications, and to contribute to early disease diagnosis, treatment, and tissue repair.