Wnt信号通路在突触可塑性调节中起重要作用，如调节轴突导向、树突形态发生和突触形成等，但是Wnt在学习记忆中的作用目前尚不清楚。本课题采用场景性恐惧记忆(contextual fear conditioning, CFC)作为实验模型，通过动物行为学、脑内核团埋管给药、实时定量PCR、免疫荧光染色、Western blot、病毒转染等技术，观察CFC学习记忆过程中，小鼠海马内Wnt的分泌表达及其下游信号通路的激活；探究抑制或增强海马内Wnt信号通路对CFC记忆的影响；并进一步研究Wnt通过调控哪些信号通路或机制参与调控CFC记忆的不同过程。本课题旨在使我们了解Wnt参与CFC学习记忆的神经生物学机制，并为将来以Wnt通路为靶标的记忆干预提供新思路和新方法。

Wnt signaling pathway plays critical roles in synaptic plasticity, such as axon guidance, dendritic morphogenesis and synapse formation. However, to date the role of Wnts in learning and memory is still unclear. In the present study, using the contextual fear conditioning (CFC) paradigm with combined molecular and pharmacological approaches, we aimed to investigate: 1) whether the CFC training could induce the secretion and synthesis of Wnts and trigger the activation of Wnts downstream signaling pathway in the hippocampus; 2) the role of Wnt signaling pathway in contextual fear memory formation by means of loss-of-function and gain-of-function studies; 3) whether the divergent Wnt downstream pathway play different roles in different phases of the contextual fear memory. Our study will help further understanding the role of Wnt signaling pathway in contextual fear memory and provide new target for the intervention of memory.