组织工程技术可修复大面积膀胱缺损，但工程化组织体内快速血管化问题一直未得到有效解决，限制了其进一步的临床转化和应用。组织工程血管化涉及诸多因素，本研究拟从种子细胞、支架材料和体内预制三方面进行膀胱组织工程修复快速血管化条件优选。脂肪来源干细胞(ASCs)可以向膀胱平滑肌和尿路上皮分化，同时具有促进新生血管的能力，是膀胱修复合适的种子细胞。本研究通过ASCs的低氧诱导预处理， ASCs与内皮祖细胞的体内外共培养，研究SDF-1α、CXCR4、CXCR7以及下游信号通路的调控机制；通过含促血管生长因子的碳纳米管缓释剂修饰三维多孔聚己内酯-壳聚糖支架；以及体内细胞材料复合物的预制技术，来促进组织工程膀胱修复的快速血管化。通过优化不同快速血管化条件来制定组织工程膀胱修复的具体策略，研究并阐明组织工程快速血管化过程中涉及的关键性机制，为大面积膀胱缺损修复和最终完全替代建立适合的技术条件和理论依据。

Althought tissue engineering can be used for large area bladder defect repairing, the rapid neovascularization is a key issue to be investigated. This study try to solve the problem from seeding cell, scaffold and prefabrication in vivo. The adipose derived stem cell(ASCs) can be differentiated into both smooth muscle cell and urothelium, meanwhile have robust angiogenesis ability, which is suitable for bladder regeneration. This study will use the hypoxia related pathway factors precondition and endothelial progenitor cells coculture to promote the neovascularization of tissue engineering bladder regeneration. In the study of scaffold, using the carbon nanotube embeded with sustained released angiogenic factors combine with polycaprolactone-chitosan scaffold to promote bladder vascularization. The in vivo bioreactor is another strategy for angiogenesis. We will optimize the different neovascularization conditions and explore the possible mechanism to provide a compatible technology and reasonable theory for large area bladder repair and even total bladder regeneration.