牛尾菜为我国民间广泛应用于治疗痛风、筋骨疼痛等症的常用中草药。野生资源分布广、蕴藏量大。申请者前期研究发现牛尾菜根茎中富含结构新颖的甾体皂苷类成分（达 26.5mg/g 生药），药理实验证明其对高尿酸血症小鼠具有良好的降低血清尿酸作用，且与 URAT1 蛋白表达相关。. 因此，本项目拟以 URAT1 为靶点并结合高尿酸血症小鼠体内抗高尿酸血症实验，以体内暴露物质评价为导向采用天然有机化合物分离纯化和结构鉴定方法，以期确定牛尾菜总皂苷中具有显著抗高尿酸血症活性、结构新颖的活性成分，发现新的药物资源。并研究活性成分对尿酸生成关键酶XOD的影响和对靶点 URAT1 的上游通路如 SLC22A12、SLC2A9 及 Pdzk1 等的作用，籍以探讨其抗高尿酸血症的作用机制，为应用现代科学实验阐释其功效记载，为开发高效、安全的新型抗高尿酸血症药物奠定基础。

Smilax riparia, a common Chinese herbs with widespread wide resources and large reserves, is widely used for treatment of gout and arthralgiaandmyalgia in folk. Pilot studies sponsored by the applicant revealed that its rhizome is rich in ingredient of steroid saponin with a concentration of as high as 26.5 mg/g crude drug. As a pharmacological test performed on acute hyperuricemia model mice showed, being characteristic of new structure, this ingredient is well active to reduce UA, which is also relative to URAT1 protein expression..Based on the pilot studies, this project is aimed to addressed the following items: to confirm such an active ingredient which is significant active to resist hyperuricemia with a new structure, and try to discover a new medicinal resource; to study the impacts on upstream access of XOD and URAT1 (such as SLC22A12, SLC2A9 and Pdzk1) from this new active ingredient, explore the action mechanism of hyperuricemia resisting, and try to lay a foundation for developing new-type, efficient and safety anti-hyperuricemia medicines. Above items have been achieved via conducting following tests and methods: targeting URAT1 and combining anti-hyperuricemia experiments conducted on hyperuricemia model mice, directed by estimations to materials exploring, isolation and purification methods to natural organic compounds and structure identification methods were employed, respectively.