eIF4A基因在水稻条纹病毒侵染过程中的作用机制研究

水稻条纹病毒（rice stripe virus, RSV）是由灰飞虱传播的一种重要水稻病毒。我们在前期研究中发现植物eIF4A基因在RSV侵染后表达下调，进一步分析发现来源于RSV的一条vsiRNA可靶向作用于该基因、调控其表达；而eIF4A基因沉默导致植物叶片皱缩和植株矮缩，表明该基因的下调参与RSV的症状表现。有趣的是，我们发现部分超表达eIF4A基因的植株表现出对RSV的抗性，表明该基因除了受RSV vsiRNA调控之外，可能在RSV与植物互作过程中还发挥其他功能。本项目拟此基础上，利用eIF4A基因超表达材料和基因编辑材料，通过转录组、蛋白质组、蛋白质互作等分析eIF4A基因调控的下游途径，并验证它们在eIF4A影响RSV积累过程中的作用，明确eIF4A基因在RSV侵染过程中的作用机制。项目的开展对于深入了解RSV与寄主互作、了解植物抗病毒途径有一定的理论价值。

Rice stripe virus (RSV), transmitted by the small brown planthopper (SBPH; Laodelphax striatellus Fallén), causes serious epidemics in East Asia, including China, Japan and Korea. In our previous report, we found that eIF4A was downregulated upon RSV infection. Further research revealed that an siRNA derived from RSV RNAs targetted eIF4A for regulation. Silencing of eIF4A caused Nicotiana benthamiana plants leaf-twisting and stunting that was visible on RSV-infected N. benthamiana. Interestingly, the eIF4A-overexpressed plants showed the attenuated symptom or nonsymptom, and had significantly lower levels of RSV RNAs than RSV-infected wild type plants. It suggests that eIF4A might have potential functions during RSV infection. Here, to uncover the function of eIF4A, we plan to use the eIF4A-overexpressed plants and eIF4A-edited plants to identify the pathway regulated by eIF4A, which is benefit to understand the interaction between RSV and plants and the resistant mechanism of plants against RSV.