印记基因对家畜胚胎发育及出生后的生长速度、瘦肉率、产肉量等数量性状有极大影响。因此，在猪中鉴定印记基因并分析其功能和表达调控机制，对于家畜育种有重要意义。申请人前期研究表明反转录转座子1（RTL1）基因在猪中表现为母本印记，并且与成肌细胞分化有关，本项目以猪胚胎不同发育阶段的肌肉组织和不同分化时期的骨骼肌卫星细胞为试验材料，利用亚硫酸氢盐直接测序、Realtime RT-PCR、Western blot、EMSA和ChIP等试验方法，分析RTL1基因启动子的差异甲基化区（DMR）甲基化状态与其印记状态及表达水平的关系；同时，研究启动子区SNPs与猪生产性状的关系。本研究有助于揭示DNA甲基化对RTL1基因的表达调控机制，为印记基因应用于分子育种奠定基础。

Imprinted genes have a significant effect on livestock embryo development and postnatal growth rate, lean meat, meat yields and other quantitative traits. Therefore, identifying more imprinted genes in pigs and analyzing their function and regulation mechanisms have great significance for livestock breeding. This project chose the muscle tissues of porcine embryos at different developmental stages and skeletal muscle satellite cells of different differentiation period as experimental materials, the methods bisulfite sequencing PCR, Realtime RT-PCR, Western blot, EMSA and ChIP are used, to analyze the relationship between RTL1 promoter differentially methylated region (DMR), and its impriting status and expression levels. Meanwhile, identify the relationship between promter SNPs and the porcine production traits. This study is helpful to reveal the regulation mechanism of methylation to RTL1 gene, and lay the foundation for the application of imprinted gene in molecular breeding.