新型生物刺激响应性纳米载药体系在激光诱导下治疗鼻咽癌的研究

世界约80%鼻咽癌发生在中国，是我国重点防治的恶性肿瘤之一。本项目在材料与生物医学交叉研究前沿和我们前期研究基础上，以具有石墨烯壳层结构且兼具优异光热转化和高效载药能力的新型水溶性碳纳米笼和谷胱甘肽刺激响应性的生物可降解聚合物为载体，包埋兼具优异光热治疗和光动力治疗双重功效的叶绿素铜钠、鼻咽癌常用的联合化疗药物顺铂/5-氟尿嘧啶，并与新型多肽连接，构建能在肿瘤特殊环境响应释药、并且能通过静脉注射高效靶向送药和瘤中直接给药配合应用的两种新型纳米载药粒子，以低功率近红外激光为综合治疗的控制开关，诱导肿瘤中载药粒子发挥"热疗+光动力治疗+化疗"的协同增效治疗作用，以安全、靶向、高效、可控方式在癌细胞内高效摧毁鼻咽癌肿瘤。 本项目克服了临床常用的放疗与化疗治疗鼻咽癌存在的不足，在纳米治疗试剂结构与性能以及治疗策略上有突出创新，在治疗水平上有重要突破，研究成果对于促进鼻咽癌治疗技术的发展，意义重大。

About 80 % nasopharyngeal cancer (NPC) patients all over the world are in China. NPC is one of the malignant tumors which are key prevented and treated by Chinese. In this project, a novel water-soluble carbon nanocage (CNC) both with photothermal conversion and drug loading properties, as well as a glutathione stimuli-sensitive polymer, will be used for encapsulating sodium copper chlorophyllin, cis-Dichlorodiamineplatinum(II) and 5-Fluorouracil for constructing two types of drug-loaded nanoparticles, which are based on our previous work and the advanced interdisciplinary studies of materials and biomedicine. The two types of drug-loaded nanoparticles obtained will be then conjugated with a novel peptide for recognizing NPC cells. After the two nano-bioconjugations are intratumorally injected into mouse and injected via tail vein, respectively, a near-infrared laser light with low power density will be used for irradiating the mouse tumors. The nano-bioconjugations inside tumors will release CNC and drugs due to the reductive environment of tumor, and will exhibit "photothermal + photodynamic + chemotherapis" upon laser irradiation. This is a safe, targeting, high efficient and controllable strategy for NPC therapy. The method described in this project has more advantage compared with the traditional technique used in clinic. Great innovations can be found in the nano-therapy reagent structures and properties, as well as in the therapy strategy. The traditional NPC therapy technique will be improved by the achievements presented in this research project.