由灰飞虱传播的水稻黑条矮缩病毒（RBSDV）严重危害我国水稻和玉米生产，而已有研究表明自噬在病毒侵染和复制及介体传毒中具有重要作用。申请人前期研究结果表明RBSDV侵染引起传毒介体灰飞虱细胞自噬，但自噬在灰飞虱与RBSDV互作中的作用机制亟待揭示。鉴于植物昆虫细胞自噬与病毒关系的研究现状及自噬在病毒侵染中的重要性和复杂性，本项目拟通过免疫荧光、激光共聚焦显微镜和电镜技术、基因表达和沉默、酵母双杂交、GST pull-down等技术分析鉴定RBSDV及其编码蛋白诱导灰飞虱细胞自噬，通过药物和RNAi沉默激活或抑制自噬来分析灰飞虱自噬对RBSDV侵染、复制和介体传毒的影响，分离鉴定与病毒自噬诱导蛋白互作的灰飞虱自噬相关蛋白2-3个，并揭示蛋白互作的生化机制和生物学意义，解析灰飞虱细胞自噬在RBSDV侵染、复制及介体传毒中的作用机制，从而为阐明灰飞虱传播RBSDV机制提供新的理论依据。

Rice black-streaked dwarf virus (RBSDV) transmitted by small brown planthopper (SBPH) severely harms rice and maize production in China. Previous researches indicate that the cell autophagy plays an important role in virus infection, replication and vector transmission. The result of the applicant's previous work indicated that RBSDV infection caused cell autophagy in SBPH vector. However, the role and mechanism of autophagy in interaction between SBPH and RBSDV urgently needs to be uncovered. In view of the research status of the relationship between plant insect cell autophagy and virus, and the importance and complexity of autophagy in virus infection, this project plans to prove that RBSDV and its encoded protein induce cell autophagy in SBPH, analyze the effect of SBPH autophagy on virus infection, replication, and vector transmission using drugs and RNAi silencing to activate or inhibit autophagy, isolate and identify 2-3 SBPH autophagy-related proteins interacting with the viral proteins, reveal the biochemical mechanism and biological significance of the protein-protein interaction, elucidate the role and mechanism of SBPH autophagy in RBSDV infection, replication and vector transmission by technologies such as immunofluorescence, laser confocal microscopy and electron microscopy, gene expression and silencing, yeast two-hybrid assay, qRT-PCR, Western blot, GST pull-down. The research results will provide new theory for elucidating the mechanism of RBSDV vector transmission.