甲磺酰菌唑是本研究团队开发新型高效仿生杀菌剂。由可可毛色二孢菌引起的茶树叶斑病是由本团队新发现的茶树叶部病害。该病害发生面广、成灾重、防控难度大，亟待研究防控技术和措施。本项目拟在评价甲磺酰菌唑对可可毛色二孢菌抑菌活性的基础上，采用甾醇含量及电导率测定、扫描电镜和透射电镜形态学观察、呼吸链NADH等重要酶活性测定、及DNA、RNA和蛋白质大分子合成评价等经典生物合成和能量抑制的方法，评价甲磺酰菌唑对可可毛色二孢菌的作用机制。综合转录组、翻译组结合生物信息学方法评价甲磺酰菌唑的候选靶标分子，采用ITC、MST和结晶衍射等方法研究甲磺酰菌唑与候选靶标分子间的相互作用。对甲磺酰菌唑官能团分子进一步衍生修饰及候选靶标分子的突变位点改造，进行活性和机制的验证。本项目有望发现甲磺酰菌唑的抑菌机制和靶标蛋白，为高活性杀菌剂创制及茶树病害田间防控应用奠定基础。

Jiahuangxianjunzuo (JHXJZ) is a new type of bionic fungicide with higher efficiency, which was developed by our research team. Tea leaf spot caused by Lasiodiplodia theobromae was a new disease in tea plant (Camellia sinensis), which was firstly discovered by our team and in urgent need of measures for preventing and controlling because of higher occurrence area and huge damage. In this research, the anti-fungal activity of JHXJZ against L. theobromae was evaluated, and the classical method of biosynthesis and energy suppression was used to study the action mechanism of JHXJZ, such as the determination of sterol content and electrical conductivity, morphological observation using scanning electron microscope and transmission electron microscope, the determination activity of NADH located in respiratory chain, and the evaluation of bio-synthesis of DNA, RNA and protein. Integrated transcriptome and translatome coupled with bio-informatics were evaluated candidate target, then interaction between JHXJZ and candidate target was studied using ITC, MST and crystalline diffraction. The anti-fungal activity and mechanism were further verified though derivative modification against functional group of JHXJZ and mutant modification of candidate target. Our studies would contribute to determine action mechanism and target protein in order to lay the road for fungicides development of higher efficiency and field application against tea plant disease.